A therapeutic angiogenesis of sustained release of basic fibroblast growth factor using biodegradable gelatin hydrogel sheets in a canine chronic myocardial infarction model.

Abstract

This study investigated the safety and efficacy of a sustained release of basic fibroblast growth factor (bFGF) with biodegradable gelatin hydrogel sheets as therapeutic angiogenesis in canine chronic myocardial infarction (MI) models. Canine chronic MI model was induced by ligating the left anterior descending coronary artery and its diagonal branches. At 4 week post-induction, we applied either saline (Control group, n = 5) or 200 μg of bFGF (Treatment group, n = 6) soaked gelatin hydrogel sheets on the ischemic area of the left ventricular (LV) wall. At 6 weeks after the procedure, we evaluated the efficacy by echocardiography and immunohistochemical study. There were no procedure-related adverse events or deaths. The serum bFGF level was under detectable levels in all animals at any sampling points. In terms of efficacy, echocardiographic evaluation demonstrated that fractional shortening was significantly improved in the treatment group. In addition, immunohistochemical study showed that the capillary density in the border zone of the MI area, as well as the MI area, significantly increased in the treatment group. Therapeutic angiogenesis by bFGF using biodegradable gelatin hydrogel sheets was safe, increased the capillary density, and improved LV function in canine chronic MI models.

• Authors: Motoyuki Kumagai, Kenji Minakata, Hidetoshi Masumoto, Masaya Yamamoto, Atsushi Yonezawa, Takafumi Ikeda, Kyokun Uehara, Kazuhiro Yamazaki, Tadashi Ikeda, Kazuo Matsubara, Masayuki Yokode, Akira Shimizu, Yasuhiko Tabata, Ryuzo Sakata, Kenji Minatoya
• Journal: Heart and vessels (Heart Vessels) Vol. 33 Issue 10 Pg. 1251-1257 (Oct 2018) ISSN: 1615-2573 [Electronic] Japan
• PMID: 29761379 (Publication Type: Journal Article)

Chemical References

• Delayed-Action Preparations
• Drug Implants
• Hydrogels
• Recombinant Proteins
• Fibroblast Growth Factor 2

Topics

• Animals
• Chronic Disease
• Coronary Vessels (diagnostic imaging, drug effects)
• Delayed-Action Preparations
• Disease Models, Animal
• Dogs
• Drug Implants
• Fibroblast Growth Factor 2 (administration & dosage)
• Hydrogels
• Male
• Microspheres
• Myocardial Infarction (diagnosis, drug therapy)
• Neovascularization, Pathologic (diagnosis, drug therapy)
• Recombinant Proteins