Effects of long non-coding RNA URHC on proliferation, apoptosis and invasion of colorectal cancer cells.

Abstract

OBJECTIVE:

To investigate the effect of long non-coding RNA URHC on the proliferation, apoptosis and invasion of colorectal cancer cells.

PATIENTS AND METHODS:

The expression of lncRNA-URHC in tissues and cells was tested by Real-time quantitative PCR. The expression of lncRNA-URHC was down-regulated by RNA interference (siRNA). The Real-time quantitative polymerase chain reaction (PCR) method was used to detect the interference efficiency. Cell counting kit-8 (CCK-8), flow cytometry, and transwell were used to detect the effect of lncRNA-URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. The effect of lncRNA-URHC on epithelial-mesenchymal transition (EMT)-related markers was detected by Western blot.

RESULTS:

LncRNA-URHC expression was significantly increased in colorectal cancer cells compared with normal cells, and the expression of lncRNA-URHC in colorectal cancer cells was higher than that in the normal cell. After down-regulated the expression of lncRNA-URHC, the proliferation and invasion of colorectal cancer cells were decreased, while cells apoptosis was promoted. Down-regulation of lncRNA-URHC could enhance the expression of E-cadherin and reduce the expression of N-cadherin, vimentin and snail.

CONCLUSIONS:

Down-regulation of lncRNA-URHC can inhibit the progression of colorectal cancer.

• Authors: Z-G Gu, G-H Shen, J-H Lang, W-X Huang, Z-H Qian, J Qiu
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 22 Issue 6 Pg. 1658-1664 (03 2018) ISSN: 2284-0729 [Electronic] Italy
• PMID: 29630109 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• Antigens, CD
• CDH1 protein, human
• Cadherins
• RNA, Long Noncoding
• RNA, Small Interfering
• Vimentin

Topics

• Antigens, CD (metabolism)
• Apoptosis (genetics)
• Cadherins (metabolism)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Colorectal Neoplasms (genetics, metabolism)
• Down-Regulation
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Humans
• Male
• RNA Interference
• RNA, Long Noncoding (genetics)
• RNA, Small Interfering (genetics)
• Vimentin (metabolism)