Abstract |
Regeneration, relying mainly on resident adult stem cells, is widespread. However, the mechanism by which stem cells initiate proliferation during this process in vivo is unclear. Using planarian as a model, we screened 46 transcripts showing potential function in the regulation of local stem cell proliferation following 48 h regeneration. By analyzing the regeneration defects and the mitotic activity of animals under administration of RNA interference (RNAi), we identified factor for initiating regeneration 1 (Fir1) required for local proliferation. Our findings reveal that Fir1, enriched in neoblasts, promotes planarian regeneration in any tissue-missing context. Further, we demonstrate that DIS3 like 3'-5' exoribonuclease 2 (Dis3l2) is required for Fir1 phenotype. Besides, RNAi knockdown of Fir1 causes a decrease of neoblast wound response genes following amputation. These findings suggest that Fir1 recognizes regenerative signals and promotes DIS3L2 proteins to trigger neoblast proliferation following amputation and provide a mechanism critical for stem cell response to injury.
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Authors | Xiao-Shuai Han, Chen Wang, Fang-Hao Guo, Shuang Huang, Yong-Wen Qin, Xian-Xian Zhao, Qing Jing |
Journal | Protein & cell
(Protein Cell)
Vol. 10
Issue 1
Pg. 43-59
(01 2019)
ISSN: 1674-8018 [Electronic] Germany |
PMID | 29557542
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Helminth Proteins
- Ribonucleases
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Topics |
- Animals
- Cell Proliferation
- Helminth Proteins
(genetics, physiology)
- Models, Animal
- Planarians
(cytology, genetics, physiology)
- RNA Interference
- Regeneration
- Ribonucleases
(metabolism)
- Stem Cells
(cytology)
- Zinc Fingers
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