Study on the sensitivity of primary tumor cells of patients with cervical cancer to chemotherapeutic combinations.

Abstract

PURPOSE:

To study the sensitivity of primary tumor cells from patients with cervical cancer to frequently used chemotherapeutic combinations.

METHODS:

54 samples from cervical cancer patients were collected and used to develop cell culture in vitro. Eight frequently used chemotherapeutic combinations (taxol, taxol+cisplatin, taxol+carboplatin, topotecan+cisplatin, gemcitabine+cisplatin, docetaxel, gemcitabine, cisplatin+c yclophosphamide+doxorubicin) were prepared in different concentrations [200,100,50,25, and 12.5% plasma peak concentration (PPC)] and added to tumor cell culture. Adenosine triphosphate (ATP) bioluminescence assay was used to quantify cell cytotoxicity.

RESULTS:

Sensitivity of primary tumor cell from patients with cervical cancer to 8 chemotherapeutic combinations were: gemcitabine < docetaxel < topotecan+cisplatin < cisplatin +cyclophosphamide+doxorubicin < gemcitabine+cisplatin < taxol < taxol+carboplatin < taxol+cisplatin.

CONCLUSION:

Using the drug sensitivity test to assess chemotherapeutics in the treatment of cervical cancer patients offers many benefits, being a first step to a clinically individualized chemotherapy.

• Authors: Fang Qiu, Xia Zhao
• Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology (J BUON) 2018 Jan-Feb Vol. 23 Issue 1 Pg. 117-123 ISSN: 1107-0625 [Print] Cyprus
• PMID: 29552770 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Carboplatin
• Paclitaxel
• Cisplatin

Topics

• Antineoplastic Agents (pharmacology)
• Antineoplastic Combined Chemotherapy Protocols (pharmacology)
• Carboplatin (pharmacology)
• Cisplatin (pharmacology)
• Drug Screening Assays, Antitumor
• Female
• Humans
• Paclitaxel (pharmacology)
• Uterine Cervical Neoplasms (drug therapy)