Neonatal Ethanol Disturbs the Normal Maturation of Parvalbumin Interneurons Surrounded by Subsets of Perineuronal Nets in the Cerebral Cortex: Partial Reversal by Lithium.

Abstract

Reduction in parvalbumin-positive (PV+) interneurons is observed in adult mice exposed to ethanol at postnatal day 7 (P7), a late gestation fetal alcohol spectrum disorder model. To evaluate whether PV+ cells are lost, or PV expression is reduced, we quantified PV+ and associated perineuronal net (PNN)+ cell densities in barrel cortex. While PNN+ cell density was not reduced by P7 ethanol, PV cell density decreased by 25% at P90 with no decrease at P14. PNN+ cells in controls were virtually all PV+, whereas more than 20% lacked PV in ethanol-treated adult animals. P7 ethanol caused immediate apoptosis in 10% of GFP+ cells in G42 mice, which express GFP in a subset of PV+ cells, and GFP+ cell density decreased by 60% at P90 without reduction at P14. The ethanol effect on PV+ cell density was attenuated by lithium treatment at P7 or at P14-28. Thus, reduced PV+ cell density may be caused by disrupted cell maturation, in addition to acute apoptosis. This effect may be regionally specific: in the dentate gyrus, P7 ethanol reduced PV+ cell density by 70% at P14 and both PV+ and PNN+ cell densities by 50% at P90, and delayed lithium did not alleviate ethanol's effect.

• Authors: Mariko Saito, John F Smiley, Maria Hui, Kurt Masiello, Judith Betz, Maria Ilina, Mitsuo Saito, Donald A Wilson
• Journal: Cerebral cortex (New York, N.Y. : 1991) (Cereb Cortex) Vol. 29 Issue 4 Pg. 1383-1397 (04 01 2019) ISSN: 1460-2199 [Electronic] United States
• PMID: 29462278 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © The Author(s) 2018. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: [email protected].

Chemical References

• Parvalbumins
• Ethanol
• Lithium

Topics

• Animals
• Apoptosis (drug effects)
• Cell Count
• Dentate Gyrus (drug effects, growth & development)
• Ethanol (administration & dosage)
• Extracellular Matrix (drug effects, pathology)
• Female
• Interneurons (chemistry, drug effects, pathology)
• Lithium (administration & dosage)
• Male
• Mice, Inbred C57BL
• Parvalbumins (analysis)
• Somatosensory Cortex (drug effects, growth & development)