This study was performed to investigate the possible involvement of
polyamines in the development of
cervical cancer. The objective of the present study was therefore to find the specific
polyamine indicators, which could be used as useful markers for the early determination of
cervical cancer. A simple method for the simultaneous determination of plasma concentrations of five
polyamines in normal and U14 model mice was developed by using HPLC-MS. The samples were derivatized by
benzoyl chloride. The derived
polyamines were separated on a C18 column by a gradient elution with
methanol-water, and then detected with HPLC-MS. The results showed that all
polyamine levels in the U14 model mice were higher than those in normal ones. The
cadaverine,
putrescine and 1, 3-diaminopropane levels were significantly higher in U14 model mice plasma than those in normal mice plasma, especially the
putrescine and 1, 3-diaminopropane (P<0.01). The
cadaverine,
putrescine and 1, 3-diaminopropane levels were significantly higher in U14 mice model urine than those in normal mice urine, especially the
cadaverine and 1, 3-diaminopropane (P<0.01).
Putrescine,
cadaverine and 1, 3-diaminopropane might be the indicators of the
cervical cancer.