Vibrio vulnificus VvhA induces Th1 and Tfh cells to proliferate against Vibrio vulnificus in a mouse model of infection.

Abstract

AIM:

To characterize the roles of VvhA in host's acquired immune response to Vibrio vulnificus infection.

MATERIALS & METHODS:

The recombinant VvhA fusion protein was used to immunize mice and the anti-VvhA polyclonal antibody was produced in vivo for prophylactic and therapeutic efficacy assay. The roles of VvhA in T helper (Th) cells differentiation were analyzed by vvhA-deleted mutant during the early phase of infection, while the ratio of Th2 and T follicular helper (Tfh) cells were examined in VvhA immunization.

RESULTS:

Anti-VvhA antibody exhibited neutralization activity against V. vulnificus. Wild-type strain induced higher level of Th1 cells than the mutant, and the concentrations of IgG2a and IFN-γ were increased during the early phase of infection. The spontaneous development of Tfh was observed in immunized model, and the serum IL-21 was increased.

CONCLUSION:

V. vulnificus VvhA elicited cellular and humoral immune responses by Th1 and Tfh cells to provide protection against VvhA.

• Authors: Ming-Yi Wang, Xiao-Fei Liu, Jun Xia, Yu Li, Jian-Li Geng, Cheng-Jin Hu
• Journal: Future microbiology (Future Microbiol) Vol. 12 Pg. 953-965 (09 2017) ISSN: 1746-0921 [Electronic] England
• PMID: 28816516 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Proteins
• Cytokines
• IL10 protein, mouse
• Immunoglobulin G
• Interleukins
• Recombinant Proteins
• VvhA protein, Vibrio vulnificus
• Interleukin-10
• Interferon-gamma
• interleukin-21

Topics

• Animals
• Antibodies, Bacterial (immunology, therapeutic use)
• Antigens, Bacterial (genetics, immunology)
• Bacterial Proteins (genetics, immunology, physiology, toxicity)
• Cell Differentiation (immunology)
• Cell Proliferation
• Cytokines (blood)
• Disease Models, Animal
• Female
• Gene Expression Regulation, Bacterial
• Genes, Bacterial (genetics)
• Genetic Vectors
• Immunity, Humoral
• Immunization
• Immunoglobulin G (blood)
• Interferon-gamma (blood)
• Interleukin-10 (blood)
• Interleukins (blood)
• Mice
• Mice, Inbred BALB C
• Recombinant Proteins
• Sequence Deletion
• Survival Rate
• T-Lymphocytes, Helper-Inducer (immunology)
• Th1 Cells (immunology)
• Th17 Cells (immunology)
• Th2 Cells (immunology)
• Vibrio Infections (immunology, metabolism, prevention & control)
• Vibrio vulnificus (genetics, metabolism)