Serum amyloid A enrichment impairs the anti-inflammatory ability of HDL from diabetic nephropathy patients.

Abstract

AIMS:

Impaired anti-inflammatory ability of high-density lipoprotein (HDL) has been demonstrated in patients with type-2 diabetes mellitus (T2DM). However, whether HDL from patients with diabetic nephropathy (DN) suffers additional damage remains unknown. This study compared the anti-inflammatory capacities of HDL from healthy controls, T2DM patients with normal renal function, and T2DM patients with DN.

MATERIALS AND METHODS:

HDL was isolated from healthy controls (n=33) and T2DM patients with normal renal function (n=21), chronic kidney disease (CKD) (n=27), and end-stage renal disease (ESRD) (n=27). Human peripheral blood mononuclear cells (PBMCs) from healthy volunteers were pretreated with HDL (100μg/mL) for 1h, then incubated with lipopolysaccharide (LPS) (50ng/mL) for 24h. The anti-inflammatory ability of HDL was measured as the secretion of TNF-α in LPS-activated monocytes.

RESULTS:

The anti-inflammatory ability of HDL was gradually impaired as kidney function declined. Serum amyloid A (SAA) concentration in HDLDN significantly increased and was positively correlated with the impaired anti-inflammatory ability of HDL (Pearson r=0.315, P=0.006). Furthermore, HDL supplemented with SAA significantly increased TNF-α release from PBMCs compared with that from control HDL.

CONCLUSIONS:

These findings identified an impaired anti-inflammatory capacity of HDL from DN patients, which might be attributable to SAA enrichment.

• Authors: Jing Yan Mao, Jia Teng Sun, Ke Yang, Wei Feng Shen, Lin Lu, Rui Yan Zhang, Xuemei Tong, Yan Liu
• Journal: Journal of diabetes and its complications (J Diabetes Complications) Vol. 31 Issue 10 Pg. 1538-1543 (Oct 2017) ISSN: 1873-460X [Electronic] United States
• PMID: 28760652 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier Inc. All rights reserved.

Chemical References

• Lipopolysaccharides
• Lipoproteins, HDL
• Serum Amyloid A Protein

Topics

• Adult
• Aged
• Cells, Cultured
• China (epidemiology)
• Cross-Sectional Studies
• Diabetes Mellitus, Type 2 (complications)
• Diabetic Angiopathies (epidemiology, immunology, metabolism, pathology)
• Diabetic Nephropathies (immunology, metabolism, pathology, physiopathology)
• Female
• Hospitals, University
• Humans
• Kidney (physiopathology)
• Kidney Failure, Chronic (complications, immunology, metabolism, pathology)
• Leukocytes, Mononuclear (drug effects, immunology, metabolism, pathology)
• Lipopolysaccharides (toxicity)
• Lipoproteins, HDL (blood, isolation & purification, metabolism)
• Male
• Middle Aged
• Outpatient Clinics, Hospital
• Renal Insufficiency, Chronic (complications, immunology, metabolism, pathology)
• Risk Factors
• Serum Amyloid A Protein (analysis, metabolism)
• Severity of Illness Index
• Vasculitis (complications, immunology, metabolism, pathology)