Antipodocalyxin Antibody chPcMab-47 Exerts Antitumor Activity in Mouse Xenograft Models of Colorectal Adenocarcinomas.

Abstract	Podocalyxin (PODXL) is expressed in several cancers, including brain tumors and colorectal cancers. PODXL overexpression is an independent predictor of progression, metastasis, and poor outcome. We recently immunized mice with recombinant human PODXL, which was produced using LN229 glioblastoma cells, and produced a clone PcMab-47 that could be used for investigating PODXL expression by flow cytometry and immunohistochemical analysis. Herein, we produced a human-mouse chimeric PcMab-47 (chPcMab-47) and investigated its antitumor activity against PODXL-expressing tumors. chPcMab-47 reacted with LN229, LN229/PODXL, and Chinese hamster ovary (CHO)/PODXL cells, but it did not react with CHO-K1 or PODXL-knockout LN229 cell line (PDIS-13). chPcMab-47 exerted antitumor activity against a mouse xenograft model using CHO/PODXL. Furthermore, chPcMab-47 was reactive with colorectal cancer cell lines such as HCT-15, Caco-2, HCT-8, and DLD-1. chPcMab-47 also exhibited antitumor activity against a mouse xenograft model using HCT-15. These results suggest that chPcMab-47 could be useful for antibody therapy against PODXL-expressing cancers.
Authors	Mika K Kaneko, Akiko Kunita, Shinji Yamada, Takuro Nakamura, Miyuki Yanaka, Noriko Saidoh, Yao-Wen Chang, Saori Handa, Satoshi Ogasawara, Tomokazu Ohishi, Shinji Abe, Shunsuke Itai, Hiroyuki Harada, Manabu Kawada, Yasuhiko Nishioka, Masashi Fukayama, Yukinari Kato
Journal	Monoclonal antibodies in immunodiagnosis and immunotherapy (Monoclon Antib Immunodiagn Immunother) Vol. 36 Issue 4 Pg. 157-162 (Aug 2017) ISSN: 2167-9436 [Electronic] United States
PMID	28665782 (Publication Type: Journal Article)
Chemical References	Antibodies, Monoclonal, Humanized Antibodies, Monoclonal, Murine-Derived Antineoplastic Agents, Immunological Recombinant Fusion Proteins Sialoglycoproteins podocalyxin
Topics	Adenocarcinoma (drug therapy, pathology) Animals Antibodies, Monoclonal, Humanized (chemistry, pharmacology) Antibodies, Monoclonal, Murine-Derived (chemistry, pharmacology) Antibody Specificity Antineoplastic Agents, Immunological (chemistry, pharmacology) CHO Cells Caco-2 Cells Colorectal Neoplasms (drug therapy, pathology) Cricetulus Humans Mice, Nude Protein Binding Recombinant Fusion Proteins (chemistry, pharmacology) Sensitivity and Specificity Sialoglycoproteins (immunology) Tumor Burden (drug effects) Xenograft Model Antitumor Assays

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