Bioluminescent Turn-On Probe for Sensing Hypochlorite in Vitro and in Tumors.

Abstract

Hypochlorite (ClO-) is one of the most important reactive oxygen species but using a BL probe for its selective detection (or imaging) still remains challenging. Herein, we report a latent BL probe benzoylhydrazine luciferin (1) for highly selective detection of ClO- in vitro and imaging ClO- in living cells and tumors. In vitro tests indicated that 1 could be applied for highly selective detection of ClO- within the range of 0-62.5 μM with a limit of detection of 0.705 μM. Using these unique features of 1, we successfully applied it to image ClO- in living cells and tumors. We envision that probe 1 might be applied to elucidate the biological roles of ClO- in wider physiological and pathological processes in the near future.

• Authors: Peiyao Chen, Zhen Zheng, Yunxia Zhu, Yu Dong, Fuqiang Wang, Gaolin Liang
• Journal: Analytical chemistry (Anal Chem) Vol. 89 Issue 11 Pg. 5693-5696 (06 06 2017) ISSN: 1520-6882 [Electronic] United States
• PMID: 28485134 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydrazines
• Luminescent Agents
• Oxidants
• Firefly Luciferin
• Hypochlorous Acid
• benzoylhydrazine

Topics

• Firefly Luciferin
• Hydrazines
• Hypochlorous Acid (analysis)
• Limit of Detection
• Luminescent Agents
• Luminescent Measurements (methods)
• Molecular Imaging (methods)
• Neoplasms (chemistry)
• Oxidants