Modified Metformin as a More Potent Anticancer Drug: Mitochondrial Inhibition, Redox Signaling, Antiproliferative Effects and Future EPR Studies.

Abstract

Metformin, one of the most widely prescribed antidiabetic drugs in the world, is being repurposed as a potential drug in cancer treatment. Epidemiological studies suggest that metformin exerts anticancer effects in diabetic patients with pancreatic cancer. However, at typical antidiabetic doses the bioavailability of metformin is presumably too low to exert antitumor effects. Thus, more potent analogs of metformin are needed in order to increase its anticancer efficacy. To this end, a new class of mitochondria-targeted metformin analogs (or mito-metformins) containing a positively-charged lipophilic triphenylphosphonium group was synthesized and tested for their antitumor efficacy in pancreatic cancer cells. Results indicate that the lead compound, mito-metformin10, was nearly 1000-fold more potent than metformin in inhibiting mitochondrial complex I activity, inducing reactive oxygen species (superoxide and hydrogen peroxide) that stimulate redox signaling mechanisms, including the activation of adenosinemonophosphate kinase and inhibition of proliferation of pancreatic cancer cells. The potential use of the low-temperature electron paramagnetic resonance technique in assessing the role of mitochondrial complexes including complex I in tumor regression in response to metformin and mito-metformins in the in vivo setting is discussed.

• Authors: Balaraman Kalyanaraman, Gang Cheng, Micael Hardy, Olivier Ouari, Adam Sikora, Jacek Zielonka, Michael B Dwinell
• Journal: Cell biochemistry and biophysics (Cell Biochem Biophys) Vol. 75 Issue 3-4 Pg. 311-317 (Dec 2017) ISSN: 1559-0283 [Electronic] United States
• PMID: 28429253 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Reactive Oxygen Species
• Metformin
• Hydrogen Peroxide
• Electron Transport Complex I

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Electron Spin Resonance Spectroscopy
• Electron Transport Complex I (metabolism)
• Humans
• Hydrogen Peroxide (chemistry, metabolism)
• Metformin (pharmacology)
• Mitochondria (chemistry, drug effects, metabolism)
• Oxidation-Reduction
• Pancreatic Neoplasms (metabolism, pathology)
• Reactive Oxygen Species (metabolism)
• Signal Transduction (drug effects)