Anti-proliferative effect of novel primary cetyl alcohol derived sophorolipids against human cervical cancer cells HeLa.

Abstract

Sophorolipids (SLs) are glycolipid biosurfactants that have been shown to display anticancer activity. In the present study, we report anti-proliferative studies on purified forms of novel SLs synthesized using cetyl alcohol as the substrate (referred as SLCA) and their anticancer mechanism in human cervical cancer cells. Antiproliferative effect of column purified SLCA fractions (A, B, C, D, E and F) was examined in panel of human cancer cell lines as well as primary cells. Among these fractions, SLCA B and C significantly inhibited the survival of HeLa and HCT 116 cells without affecting the viability of normal human umbilical vein endothelial cells (HUVEC). The two fractions were identified as cetyl alcohol sophorolipids with non-hydroxylated tail differing in the degree of acetylation on sophorose head group. At an IC50 concentration SLCA B (16.32 μg ml-1) and SLCA C (14.14 μg ml-1) blocked the cell cycle progression of HeLa cells at G1/S phase in time-dependent manner. Moreover, SLCA B and SLCA C induced apoptosis in HeLa cells through an increase in intracellular Ca2+ leading to depolarization of mitochondrial membrane potential and increase in the caspase-3, -8 and -9 activity. All these findings suggest that these SLCAs could be explored for their chemopreventive potential in cervical cancer.

• Authors: Laxman Nawale, Parul Dubey, Bhushan Chaudhari, Dhiman Sarkar, Asmita Prabhune
• Journal: PloS one (PLoS One) Vol. 12 Issue 4 Pg. e0174241 ( 2017) ISSN: 1932-6203 [Electronic] United States
• PMID: 28419101 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Fatty Alcohols
• Glycolipids
• Surface-Active Agents
• cetyl alcohol
• Caspases
• Calcium

Topics

• A549 Cells
• Antineoplastic Agents (chemistry, pharmacology)
• Apoptosis (drug effects)
• Calcium (metabolism)
• Caspases (metabolism)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Dose-Response Relationship, Drug
• Fatty Alcohols (chemistry, pharmacology)
• Female
• G1 Phase Cell Cycle Checkpoints (drug effects)
• Glycolipids (chemistry, pharmacology)
• HCT116 Cells
• HeLa Cells
• Human Umbilical Vein Endothelial Cells (cytology, drug effects)
• Humans
• MCF-7 Cells
• Membrane Potential, Mitochondrial (drug effects)
• Microscopy, Confocal
• Surface-Active Agents (chemistry, pharmacology)
• Time Factors
• Uterine Cervical Neoplasms (metabolism, pathology)