Abstract |
Alzheimer's disease (AD) is a chronic and multi-factor-induced neurodegenerative disorder. The development of a single-target treatment strategy for AD has not been successful. Shengmai-San (SMS), a traditional Chinese medicine containing multi-active components, has been reported to be effective for treating AD. However, the mechanism of SMS is still unclear, and as a multicomponent combination therapy, there is a lack of appropriate evaluation. Thereby, metabolomics was applied to investigate the multi-target intervention of SMS by monitoring the fold changes of metabolites in serum. An AD model was successfully replicated by treating rats with oral aluminum chloride ( AlCl3) and an intraperitoneal injection of d-galactose (d-gal) for 105 days. The intervention group was treated with SMS during the whole modeling process. The results of classical experiments showed that SMS could alleviate Aβ1-40 deposition, protect neurons in the CA3 region of the hippocampus and improve spatial learning and memory impairment compared with the model group. Based on these curative effects, a metabolomics study was implemented using the UPLC-Q/TOF-MS method. A heatmap of AD-associated metabolites (P < 0.05) was set up to target the most relevant metabolic network and to evaluate the comprehensive effects of SMS. Based on this AD-associated metabolic network, the total regulation-of-metabolites' coverage rate of SMS was 51.35% and the gross metabolites' recovery proportion was 72.32%. Lipid peroxidation was the main mechanism of SMS intervention in AD, including inhibition of the generation of linoleic acid hydroperoxides, such as 13-HPODE, 9-HPODE and 9-OxoODE.
|
Authors | Shengwen Lu, Ying Han, Hang Chu, Ling Kong, Aihua Zhang, Guangli Yan, Hui Sun, Ping Wang, Xijun Wang |
Journal | Food & function
(Food Funct)
Vol. 8
Issue 4
Pg. 1660-1671
(Apr 19 2017)
ISSN: 2042-650X [Electronic] England |
PMID | 28379235
(Publication Type: Journal Article)
|
Chemical References |
- Drug Combinations
- Drugs, Chinese Herbal
- fructus schizandrae, radix ginseng, radix ophiopogonis drug combination
|
Topics |
- Alzheimer Disease
(blood, drug therapy)
- Animals
- Chromatography, High Pressure Liquid
- Drug Combinations
- Drugs, Chinese Herbal
(administration & dosage)
- Humans
- Male
- Metabolomics
- Rats
- Rats, Wistar
- Spectrometry, Mass, Electrospray Ionization
|