Characterization and Potential Antitumor Activity of Polysaccharide from Gracilariopsis lemaneiformis.

Abstract

Substances with valuable antitumor properties have been identified in many marine algae, including an edible polysaccharide from the marine alga Gracilariopsis lemaneiformis (PGL). We previously reported transcriptome profiling data showing that PGL induced transcriptional alterations generate anti-lung cancer activity. To identify how PGL is detrimental to tumors, we purified PGL to characterize its chemical composition, molecular weight, and sugar and protein content and investigated its antitumor activity. We demonstrated that PGL exerted its antitumor activities by modulating cell viability, morphology, apoptosis, and the apoptosis-related Fas/FasL signaling pathway in the human lung cancer cell line A549, the gastric cancer cell line MKN28, and the mouse melanoma cell line B16. Our data provide the first evidence that PGL inhibits cell proliferation by inducing apoptosis, which is largely mediated by Fas/FasL in cancer cells, suggesting that PGL might be a novel therapeutic agent against cancer.

• Authors: Yani Kang, Zhi-Jiang Wang, Dongsheng Xie, Xue Sun, Wenge Yang, Xiaodong Zhao, Nianjun Xu
• Journal: Marine drugs (Mar Drugs) Vol. 15 Issue 4 (Mar 29 2017) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 28353631 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Fas Ligand Protein
• Polysaccharides

Topics

• A549 Cells
• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Fas Ligand Protein (metabolism)
• Humans
• Lung Neoplasms (drug therapy, metabolism)
• Melanoma, Experimental (drug therapy, metabolism)
• Mice
• Polysaccharides (pharmacology)
• Rhodophyta (chemistry)
• Signal Transduction (drug effects)