UVA radiation augments cytotoxic activity of psoralens in melanoma cells.

Abstract

PURPOSE:

Melanoma is an aggressive form of skin cancer. The aim of the study was to evaluate the influence of UVA radiation and psoralens: 5-methoxypsoralen (5-MOP) or 8-methoxypsoralen (8-MOP) on melanoma cells viability.

MATERIALS AND METHODS:

The amelanotic C32 and melanotic COLO829 human melanoma cell lines were exposed to increasing concentrations of psoralens (0.1-100 μM) in the presence or absence of UVA radiation. Cell viability was evaluated by the WST-1 assay.

RESULTS:

We demonstrated that 8-MOP, in contrast to 5-MOP, has no cytotoxic effect on both melanoma cell lines. Simultaneous exposure of cells to 8-MOP and UVA radiation caused significant cytotoxic response in C32 cells where the EC50 value was estimated to be 131.0 μM (UVA dose: 1.3 J/cm2) and 105.3 μM (UVA dose: 2.6 J/cm2). The cytotoxicity of 5-MOP on both C32 and COLO829 cells was significantly augmented by UVA radiation - the EC50 was estimated to be 22.7 or 7.9 μM (UVA dose: 1.3 J/cm2) and 24.2 or 7.0 μM (UVA dose: 2.6 J/cm2), respectively.

CONCLUSIONS:

The demonstrated high cytotoxic response after simultaneous exposure of melanoma cells to psoralens and UVA radiation in vitro suggests the usefulness of PUVA therapy to treat melanoma in vivo.

• Authors: Dorota Wrześniok, Artur Beberok, Jakub Rok, Marcin Delijewski, Anna Hechmann, Martyna Oprzondek, Zuzanna Rzepka, Barbara Bacler-Żbikowska, Ewa Buszman
• Journal: International journal of radiation biology (Int J Radiat Biol) Vol. 93 Issue 7 Pg. 734-739 (07 2017) ISSN: 1362-3095 [Electronic] England
• PMID: 28287037 (Publication Type: Journal Article)

Chemical References

• 5-Methoxypsoralen
• Methoxsalen

Topics

• 5-Methoxypsoralen
• Cell Line, Tumor
• Cell Survival (drug effects, radiation effects)
• Dose-Response Relationship, Drug
• Dose-Response Relationship, Radiation
• Drug Synergism
• Humans
• Melanoma (drug therapy, pathology)
• Methoxsalen (administration & dosage, analogs & derivatives)
• PUVA Therapy (methods)
• Radiotherapy Dosage
• Treatment Outcome