Abstract |
Recent findings on the existence of oncogenic fusion genes in a wide array of solid tumors, including head and neck squamous cell carcinoma ( HNSCC), suggests that fusion genes have become attractive targets for cancer diagnosis and treatment. In this study, we showed for the first time that a read-through fusion gene JMJD7-PLA2G4B is presented in HNSCC, splicing neighboring jumonji domain containing 7 (JMJD7) and phospholipase A2, group IVB (PLA2G4B) genes together. Ablation of JMJD7-PLA2G4B significantly inhibited proliferation of HNSCC cells by promoting G1 cell cycle arrest and increased starvation-induced cell death compared to JMJD7-only knockdown HNSCC cells. Mechanistically, we found that JMJD7-PLA2G4B modulates phosphorylation of Protein Kinase B (AKT) to promote HNSCC cell survival. Moreover, JMJD7-PLA2G4B also regulated an E3 ligase S-phase kinase-associated protein 2 (SKP2) to control the cell cycle progression from G1 phase to S phase by inhibiting Cyclin-dependent kinase inhibitor 1 (p21) and 1B (p27) expression. Our study provides novel insights into the oncogenic control of JMJD7-PLA2G4B in HNSCC cell proliferation and survival, and suggests that JMJD7-PLA2G4B may serve as an important therapeutic target and prognostic marker for HNSCC development and progression.
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Authors | Yingduan Cheng, Yi Wang, Jiong Li, Insoon Chang, Cun-Yu Wang |
Journal | Oncotarget
(Oncotarget)
Vol. 8
Issue 2
Pg. 1972-1982
(Jan 10 2017)
ISSN: 1949-2553 [Electronic] United States |
PMID | 28030848
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Oncogene Proteins, Fusion
- Jumonji Domain-Containing Histone Demethylases
- Oncogene Protein v-akt
- Group IV Phospholipases A2
- PLA2G4B protein, human
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Topics |
- Biomarkers, Tumor
(genetics)
- Carcinoma, Squamous Cell
(diagnosis, genetics, pathology)
- Cell Line, Tumor
- Cell Proliferation
(genetics)
- Cell Survival
(genetics)
- Group IV Phospholipases A2
(genetics, physiology)
- HCT116 Cells
- HEK293 Cells
- HT29 Cells
- Head and Neck Neoplasms
(diagnosis, genetics, pathology)
- Humans
- Jumonji Domain-Containing Histone Demethylases
(genetics, physiology)
- MCF-7 Cells
- Oncogene Protein v-akt
(metabolism)
- Oncogene Proteins, Fusion
(genetics, physiology)
- Phosphorylation
- Squamous Cell Carcinoma of Head and Neck
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