Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway.

Abstract

Tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. Our study aims to evaluate the cytotoxicity activities of T. matsutake aqueous extract (TM) in HepG2 and SMMC-7721 cells. In in vitro experiments, TM strikingly reduced cell viability, promoted cell apoptosis, inhibited cell migration ability, induced excessive generation of ROS, and caused caspases cascade and mitochondrial membrane potential dissipation in hepatocellular carcinoma cells. In in vivo experiments, 14-day TM treatment strongly suppressed tumor growth in HepG2 and SMMC-7721-xenografted nude mice without influence on their body weights and liver function. Furthermore, TM increased the levels of cleaved poly-ADP-ribose polymerase (PARP), Bad, and Bax and reduced the expressions of B-cell lymphoma 2 (Bcl-2) in treated cells and tumor tissues. All aforementioned results suggest that caspase-dependent mitochondrial apoptotic pathways are involved in TM-mediated antihepatocellular carcinoma.

• Authors: Yanzhen Wang, Yiling Chen, Xinrui Zhang, Guangsheng Cai, Shengshu An, Xue Wang, Lirong Teng, Di Wang
• Journal: BioMed research international (Biomed Res Int) Vol. 2016 Pg. 9014364 ( 2016) ISSN: 2314-6141 [Electronic] United States
• PMID: 28018916 (Publication Type: Journal Article)

Chemical References

• Neoplasm Proteins
• Plant Extracts
• Caspases

Topics

• Animals
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (drug therapy, pathology)
• Caspases (biosynthesis)
• Cell Proliferation (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Hep G2 Cells
• Humans
• Liver Neoplasms (drug therapy, pathology)
• Mice
• Mitochondria (drug effects, metabolism)
• Neoplasm Proteins (biosynthesis)
• Plant Extracts (administration & dosage, chemistry)
• Signal Transduction (drug effects)
• Tricholoma (chemistry)
• Xenograft Model Antitumor Assays