Jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to Rice black streaked dwarf virus infection in rice.

Abstract

Plant hormones play a vital role in plant immune responses. However, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot-virus defense systems. We used a high-throughput-sequencing approach to compare the global gene expression of Rice black-streaked dwarf virus (RBSDV)-infected rice plants with that of healthy plants. Exogenous hormone applications and transgenic rice were used to test RBSDV infectivity and pathogenicity. Our results revealed that the jasmonic acid (JA) pathway was induced while the brassinosteroid (BR) pathway was suppressed in infected plants. Foliar application of methyl jasmonate (MeJA) or brassinazole (BRZ) resulted in a significant reduction in RBSDV incidence, while epibrassinolide (BL) treatment increased RBSDV infection. Infection studies using coi1-13 and Go mutants demonstrated JA-mediated resistance and BR-mediated susceptibility to RBSDV infection. A mixture of MeJA and BL treatment resulted in a significant reduction in RBSDV infection compared with a single BL treatment. MeJA application efficiently suppressed the expression of BR pathway genes, and this inhibition depended on the JA coreceptor OsCOI1. Collectively, our results reveal that JA-mediated defense can suppress the BR-mediated susceptibility to RBSDV infection.

• Authors: Yuqing He, Hehong Zhang, Zongtao Sun, Junmin Li, Gaojie Hong, Qisong Zhu, Xuebiao Zhou, Stuart MacFarlane, Fei Yan, Jianping Chen
• Journal: The New phytologist (New Phytol) Vol. 214 Issue 1 Pg. 388-399 (Apr 2017) ISSN: 1469-8137 [Electronic] England
• PMID: 27976810 (Publication Type: Journal Article)
• Copyright: © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

Chemical References

• Acetates
• Brassinosteroids
• Cyclopentanes
• Oxylipins
• Plant Proteins
• jasmonic acid
• methyl jasmonate

Topics

• Acetates (pharmacology)
• Brassinosteroids (pharmacology)
• Cyclopentanes (pharmacology)
• Disease Susceptibility
• Gene Expression Regulation, Plant (drug effects)
• Genes, Plant
• Oryza (drug effects, genetics, virology)
• Oxylipins (pharmacology)
• Plant Diseases (virology)
• Plant Immunity (drug effects)
• Plant Leaves (drug effects, virology)
• Plant Proteins (genetics, metabolism)
• Plant Viruses (drug effects, physiology)
• Signal Transduction (drug effects)
• Stress, Physiological (drug effects, genetics)
• Transcription, Genetic (drug effects)