Low sensitivity of the tourniquet test for differential diagnosis of dengue: an analysis of 28,000 trials in patients.

Abstract

BACKGROUND:

The aim of this study was to evaluate the utility of the tourniquet test (TT) for dengue diagnosing. To our knowledge, no previous study with such a large sample, of this duration, with as many laboratory methods referenced, or relating the results of the TT to the 2009 WHO classification of severity has been conducted thus far.

METHODS:

In this study, we analyzed the records of 119,589 suspected dengue cases in a Brazilian city, with 30,670 confirmed cases. The Cohen's Kappa test was applied to evaluate the degree of agreement between the tests, and the sensitivity and specificity was calculated for the TT.

RESULTS:

Twenty-eight thousand six hundred thirty-five TT were performed. No association between the outcome of the TT and greater severity of infection, according to the 2009 guideline, was observed (P = 0.28); furthermore, relevant agreement with the final diagnosis (κ = 0.01; 95 % CI = 0.00 to 0.02) or individually with the IgM enzyme-linked immunoassay was not observed (κ = 0.05; 95 % CI = 0.04 to 0.06), and was even lower with PCR (κ = 0.27; 95 % CI = 0.06 to 0.49). Most importance of the TT was shown in relation to specificity (88.9 %; 95 % CI = 0.88 to 0.89) and negative predictive value (70.3 %; CI 95 % = 0.70 to 0.71).

CONCLUSIONS:

TT was more effective in detecting cases that were truly negative than positive. These results suggest that the TT should not be used as diagnosis of dengue.

• Authors: Nathália Barbosa Furlan, Caroline Tukasan, Cássia Fernanda Estofolete, Maurício Lacerda Nogueira, Natal Santos da Silva
• Journal: BMC infectious diseases (BMC Infect Dis) Vol. 16 Issue 1 Pg. 627 (Nov 03 2016) ISSN: 1471-2334 [Electronic] England
• PMID: 27809813 (Publication Type: Journal Article)

Chemical References

• Immunoglobulin M

Topics

• Adolescent
• Adult
• Brazil
• Child
• Child, Preschool
• Dengue (diagnosis, immunology)
• Dengue Virus (genetics, immunology)
• Diagnosis, Differential
• Electrophysiologic Techniques, Cardiac
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Immunoenzyme Techniques
• Immunoglobulin M (immunology)
• Infant
• Infant, Newborn
• Male
• Middle Aged
• Polymerase Chain Reaction
• Sensitivity and Specificity
• Tourniquets
• Young Adult