Abstract |
The expression of miR-143/miR-145 was up-regulated in ischemic stroke (IS), which may be used as biomarkers and/or therapeutic targets for IS. We aimed to investigate the association of rs4705342 and rs4705343 polymorphisms in the promoter of miR-143/145 with risk of IS. The study population comprised 445 patients with IS and 518 controls. The rs4705342 genotype was analyzed by using a TaqMan Assay and the rs4705343 genotype was determined by using a polymerase chain reaction-restriction fragment length polymorphism assay. Relative expression of miR-143/miR-145 was measured by quantitative real-time PCR. We found that the rs4705342 was associated with a decreased risk of IS (TC vs. TT: adjusted OR = 0.74, 95% CI, 0.57-0.97; CC vs. TT: adjusted OR = 0.53, 95% CI, 0.34-0.83). Haplotype analysis showed that the TC haplotype was associated with an increased risk of IS risk (OR = 1.33, 95% CI, 1.01-1.75), whereas the CT haplotype was associated with a decreased risk of IS risk (OR = 0.68, 95% CI, 0.50-0.92). Importantly, patients carrying the rs4705342TC/CC genotypes had a lower level of miR-145 (P = 0.03). We found for the first time that the rs4705342 CC was a protective factor for IS, probably by reducing the level of miR-145.
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Authors | Ye-Sheng Wei, Yang Xiang, Pin-Hu Liao, Jun-Li Wang, You-Fan Peng |
Journal | Scientific reports
(Sci Rep)
Vol. 6
Pg. 34620
(10 06 2016)
ISSN: 2045-2322 [Electronic] England |
PMID | 27708363
(Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MIRN143 microRNA, human
- MIRN145 microRNA, human
- MicroRNAs
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Topics |
- Aged
- Brain Ischemia
(genetics)
- Female
- Haplotypes
- Humans
- Male
- MicroRNAs
(genetics)
- Middle Aged
- Polymorphism, Restriction Fragment Length
- Promoter Regions, Genetic
- Real-Time Polymerase Chain Reaction
- Risk Factors
- Stroke
(genetics)
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