Abstract | BACKGROUND/AIM: MATERIALS AND METHODS: The cytocidal effect of G- PDT was compared to that of T- PDT as a control. Tumor viability was determined by an in vitro MTS assay. The percentage of apoptosis-positive cells was examined by triple stain flow cytometry ( annexin V, ethidium homodimer III and Hoechst 33342) in the BDC cell line (NOZ cell) in vitro. The change in transplanted tumor volume in vivo (4-week-old male BALB/c mice) was examined 7 days after PDT. RESULTS: Cell death was induced in a light dose-dependent manner by PDT. The laser power was set at 5 Jules/cm(2) to obtain half maximal inhibitory concentration (IC50) in T- PDT and G- PDT and the concentration of photosensitivity for G- PDT (2.02 μg/ml) was lower than that for T- PDT (4.14 μg/ml). Both T- PDT and G- PDT showed increased induction rates in comparison to the light only or G- chlorin only. Furthermore, the rate of apoptosis in the G- PDT (92.6%) was increased in comparison to that in the T- PDT (38.9%). The increased rates of tumor volume during the 7 days in both the G- PDT and T- PDT groups were significantly lower than that in the non- PDT group (p<0.01). At day 7, the increased rates of tumor volume in the G- PDT group were significantly lower than that in the T- PDT group (p<0.05). CONCLUSION: The new G- PDT treatment showed a high prevalence of apoptosis and inhibition of tumor growth in treatment of BDC cells.
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Authors | Goushi Murakami, Atsushi Nanashima, Takashi Nonaka, Tetsuro Tominaga, Kouki Wakata, Yorihisa Sumida, Haruo Akashi, Shigetoshi Okazaki, Hiromi Kataoka, Takeshi Nagayasu |
Journal | Anticancer research
(Anticancer Res)
Vol. 36
Issue 9
Pg. 4493-501
(09 2016)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 27630287
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
Chemical References |
- Photosensitizing Agents
- Porphyrins
- chlorin
- Glucose
- Talaporfin
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Topics |
- Animals
- Apoptosis
- Bile Duct Neoplasms
(drug therapy)
- Cell Line, Tumor
- Cell Survival
- Cholangiocarcinoma
(drug therapy)
- Flow Cytometry
- Glucose
(chemistry)
- Humans
- In Situ Nick-End Labeling
- Lasers
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Photochemotherapy
(methods)
- Photosensitizing Agents
(pharmacology)
- Porphyrins
(chemistry, pharmacology)
- Prevalence
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