Synchronization of S Phase in Schizosaccharomyces pombe Cells by Transient Exposure to M-Factor Pheromone.

Abstract

A well-characterized S phase, a unicellular lifestyle, and a plethora of mutations in key components of DNA metabolism make fission yeast a particularly attractive system in which to study DNA replication. However, synchronization of passage through a normal S phase has proved challenging. This protocol describes how combining nitrogen starvation with M-factor mating pheromone treatment presents a highly effective method for synchronizing passage through an ostensibly normal S phase.

• Authors: Olaf Nielsen
• Journal: Cold Spring Harbor protocols (Cold Spring Harb Protoc) Vol. 2016 Issue 9 (09 01 2016) ISSN: 1559-6095 [Electronic] United States
• PMID: 27587782 (Publication Type: Journal Article)
• Copyright: © 2016 Cold Spring Harbor Laboratory Press.

Chemical References

• Pheromones

Topics

• DNA Replication
• Pheromones (metabolism)
• S Phase (drug effects)
• Schizosaccharomyces (drug effects, growth & development, metabolism)