Abstract |
MicroRNA-21 (miR-21) is overexpressed in patients with rheumatoid arthritis (RA). This study was designed to investigate the effect and mechanism of miR-21 on cell proliferation in fibroblast-like synoviocytes (FLS) of RA. FLS were primary-cultured from a rat RA model. RA-FLS and normal FLS were infected with lentivirus (anti-miR-21 or pro-miR-21) for overexpression or downregulation of miR-21, respectively. The effects of miR-21 overexpression or inhibition on nucleoprotein NF-κB levels and FLS cell proliferation were evaluated by western blotting and MTT assays. The effects of an inhibitor of NF-κB nuclear translocation (BAY 11-7082) were also evaluated. The results showed that the levels of miR-21 and nucleoprotein NF-κB were increased in FLS of RA model rats compared to the control group. Downregulation of miR-21 in RA FLS led to a significant decrease in nucleoprotein NF-κB levels and cell proliferation rates compared to the antinegative control (NC) group. However, miR-21 overexpression in normal FLS resulted in a significant increase of nucleoprotein NF-κB levels and cell proliferation rates compared to the pro-NC group. The effects of miR-21 overexpression were reversed by BAY 11-7082. We concluded that upregulated miR-21 in FLS in RA model rats may promote cell proliferation by facilitating NF-κB nuclear translocation, thus affecting the NF-κB pathway.
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Authors | Ying Chen, Pei-Feng Xian, Lu Yang, Sheng-Xu Wang |
Journal | BioMed research international
(Biomed Res Int)
Vol. 2016
Pg. 9279078
( 2016)
ISSN: 2314-6141 [Electronic] United States |
PMID | 27429986
(Publication Type: Journal Article)
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Chemical References |
- MicroRNAs
- NF-kappa B
- mirn21 microRNA, rat
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Topics |
- Animals
- Arthritis, Experimental
(genetics, pathology)
- Arthritis, Rheumatoid
(genetics, pathology)
- Cell Nucleus
(metabolism)
- Cell Proliferation
- Cell Survival
- Disease Models, Animal
- Down-Regulation
(genetics)
- Fibroblasts
(metabolism, pathology)
- Male
- MicroRNAs
(genetics, metabolism)
- NF-kappa B
(metabolism)
- Protein Transport
- Rats, Wistar
- Synoviocytes
(metabolism, pathology)
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