Neutralizing Antibodies against Plasmodium falciparum Associated with Successful Cure after Drug Therapy.

Abstract

An effective antibody response can assist drug treatment to contribute to better parasite clearance in malaria patients. To examine this, sera were obtained from two groups of adult patients with acute falciparum malaria, prior to drug treatment: patients who (1) have subsequent recrudescent infection, or (2) were cured by Day 28 following treatment. Using a Plasmodium falciparum antigen library, we examined the antibody specificities in these sera. While the antibody repertoire of both sera groups was extremely broad and varied, there was a differential antibody profile between the two groups of sera. The proportion of cured patients with antibodies against EXP1, MSP3, GLURP, RAMA, SEA and EBA181 was higher than the proportion of patients with recrudescent infection. The presence of these antibodies was associated with higher odds of treatment cure. Sera containing all six antibodies impaired the invasion of P. falciparum clinical isolates into erythrocytes. These results suggest that antibodies specific against EXP1, MSP3, GLURP, RAMA, SEA and EBA181 in P. falciparum infections could assist anti-malarial drug treatment and contribute to the resolution of the malarial infection.

• Authors: Yun Shan Goh, Kaitian Peng, Wan Ni Chia, Anthony Siau, Kesinee Chotivanich, Anne-Charlotte Gruner, Peter Preiser, Mayfong Mayxay, Sasithon Pukrittayakamee, Kanlaya Sriprawat, Francois Nosten, Nicholas J White, Laurent Renia
• Journal: PloS one (PLoS One) Vol. 11 Issue 7 Pg. e0159347 ( 2016) ISSN: 1932-6203 [Electronic] United States
• PMID: 27427762 (Publication Type: Journal Article)

Chemical References

• Antibodies, Neutralizing
• Antibodies, Protozoan
• Antigens, Protozoan
• Antimalarials
• Artemisinins
• EBA-181 protein, Plasmodium falciparum
• Ethanolamines
• Fluorenes
• Immune Sera
• Protozoan Proteins
• QF116 antigen, Plasmodium falciparum
• merozoite surface protein 3, Plasmodium
• glutamate-rich protein, Plasmodium
• Azithromycin
• Artemether
• Lumefantrine

Topics

• Acute Disease
• Adolescent
• Adult
• Antibodies, Neutralizing (biosynthesis, blood)
• Antibodies, Protozoan (biosynthesis, blood)
• Antibody Specificity
• Antigens, Protozoan (blood, genetics, immunology)
• Antimalarials (therapeutic use)
• Artemether
• Artemisinins (therapeutic use)
• Azithromycin (therapeutic use)
• Cohort Studies
• Erythrocytes (drug effects, parasitology)
• Ethanolamines (therapeutic use)
• Female
• Fluorenes (therapeutic use)
• Humans
• Immune Sera (pharmacology)
• Immunity, Humoral
• Lumefantrine
• Malaria, Falciparum (blood, drug therapy, immunology, parasitology)
• Male
• Plasmodium falciparum (drug effects, growth & development)
• Protozoan Proteins (genetics, immunology)
• Recurrence
• Treatment Outcome