HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

LPLRFamide exerts short-term anorexigenic effects that coincide with magnocellular division of the hypothalamic paraventricular nucleus activation.

Abstract
LPLRFamide is a member of the RFamide peptide family that elicits an anorexigenic effect when centrally injected in chicks although the mechanism mediating this response is poorly understood. Therefore, the purpose of this experiment was to elucidate the hypothalamic mechanism of short-term anorexia after central administration of LPLRFamide in chicks. In Experiment 1 chicks centrally injected with LPLRFamide decreased food intake at 15min but not 30min following administration compared to vehicle-injected chicks. For Experiment 2, c-Fos immunoreactivity was quantified in several appetite-associated hypothalamic nuclei and in LPLRF-injected chicks, compared to vehicle-injected chicks, there was an increase in the number of reactive cells in the magnocellular division of the paraventricular nucleus. Lastly in Experiment 3, real time-PCR was performed and hypothalamic proopiomelanocortin (POMC) mRNA abundance was increased in LPRLFamide-injected chicks compared to vehicle-injected chicks. Thus, following central injection of LPLRFamide there is activation of the paraventricular nucleus of the hypothalamus and increased expression of hypothalamic POMC mRNA in chicks.
AuthorsAmy Scheel, Nick Deal, Natalie St John, Amy Wells, Maggie Caruso, Elizabeth R Gilbert, Mark Cline
JournalGeneral and comparative endocrinology (Gen Comp Endocrinol) Vol. 246 Pg. 116-119 (05 15 2017) ISSN: 1095-6840 [Electronic] United States
PMID27318277 (Publication Type: Journal Article)
CopyrightCopyright © 2016 Elsevier Inc. All rights reserved.
Chemical References
  • Oligopeptides
  • RNA, Messenger
  • leucyl-prolyl-leucyl-arginyl-phenylalaninamide
  • Pro-Opiomelanocortin
Topics
  • Animals
  • Anorexia (drug therapy, metabolism)
  • Chickens
  • Eating (drug effects)
  • Hypothalamus (drug effects, metabolism)
  • Immunoenzyme Techniques
  • Injections, Intraventricular
  • Oligopeptides (administration & dosage, pharmacology)
  • Paraventricular Hypothalamic Nucleus (drug effects, metabolism)
  • Pro-Opiomelanocortin (genetics, metabolism)
  • RNA, Messenger (genetics)
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: