N-3 polyunsaturated fatty acids attenuates triglyceride and inflammatory factors level in hfat-1 transgenic pigs.

Abstract

BACKGROUND:

The consumption of n-3 polyunsaturated fatty acids (PUFAs) is important to human health, especially in cases of cardiovascular disease. Although beneficial effects of n-3 PUFAs have been observed in a number of studies, the mechanisms involved in these effects have yet to be discovered.

METHODS:

We generated hfat-1 transgenic pigs with traditional somatic cell nuclear transfer (SCNT) technology. The fatty acid composition in ear tissue of pigs were detected with gas chromatography. The cholesterol, triglycerides (TAG) and inflammation mediators in circulation were investigated.

RESULTS:

The hfat-1 transgenic pigs were developed which accumulate high levels of n-3 PUFAs than wild-types pigs. Gas chromatography results demonstrated that the total n-3 PUFAs in the ear tissues of the transgenic founders were 2-fold higher than the wild-type pigs. A lipid analysis demonstrated that the levels of TAG in the transgenic pigs were decreased significantly. The basal levels of the inflammation mediators tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) in transgenic pigs were inhibited markedly compared with the wild-type pigs.

CONCLUSIONS:

These results suggest that n-3 PUFAs accumulation in vivo may have beneficial effects on vascular and hfat-1 transgenic pigs may be a useful tool for investigating the involved mechanisms.

• Authors: Xingxing Liu, Daxin Pang, Ting Yuan, Zhuang Li, Zhanjun Li, Mingjun Zhang, Wenzhi Ren, Hongsheng Ouyang, Xiaochun Tang
• Journal: Lipids in health and disease (Lipids Health Dis) Vol. 15 Pg. 89 (May 10 2016) ISSN: 1476-511X [Electronic] England
• PMID: 27161005 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cadherins
• Chemokine CCL2
• Cholesterol, HDL
• FAT1 protein, human
• Fatty Acids, Omega-3
• Interleukin-6
• Triglycerides
• Tumor Necrosis Factor-alpha
• Cholesterol

Topics

• Animals
• Animals, Genetically Modified
• Cadherins (genetics)
• Chemokine CCL2 (genetics)
• Cholesterol (blood, genetics)
• Cholesterol, HDL (blood, genetics)
• Fatty Acids, Omega-3 (pharmacokinetics, pharmacology)
• Female
• Humans
• Inflammation (diet therapy, genetics)
• Interleukin-6 (genetics)
• Male
• Sus scrofa
• Triglycerides (blood, genetics)
• Tumor Necrosis Factor-alpha (genetics)