Anti-Angiogenic Properties of Cafestol and Kahweol Palmitate Diterpene Esters.

Abstract

Epidemiological studies support the association of coffee-specific diterpenes, with various beneficial health effects. Although anti-antiangiogenic properties of free cafestol and kahweol have been recently described, available data regarding their esterified form, in particular palmitate esters as the main diterpene esters present in coffee, are still rare. Given that angiogenesis plays an important role in many pathological conditions, including cancer growth and metastasis, this study aimed to assess and compare the potential anti-angiogenic effects of cafestol palmitate (CP) and kahweol palmitate (KP) in an in vitro angiogenesis model. According to our findings, both compounds inhibited angiogenesis steps on human microvascular endothelial cells (HMVECs), although a more significant effect was observed for KP. Compared to control, HMVECs viability decreased in a dose-dependent manner upon incubation either with CP or KP. Concentrations of 75 and 100 μM of each compound were cytotoxic. Cell proliferation was also dramatically reduced by both diterpene esters at 50 μM, although KP had a stronger inhibitory effect. However, CP and KP did not induce apoptosis on HMVECs. Both compounds reduced cell migration, but this effect was only statistically significant after KP incubation. Inhibition of VEGFR2 expression and its downstream effector Akt, but not Erk, was also observed in CP- and KP-treated HMVECs. These findings were confirmed using ELISA assay for phosphorylated (active) VEGFR-2. Taken together, these data indicate that both CP and KP can be considered potent compounds against angiogenesis-dependent disorders. Our findings further indicate that KP exerts more potent anti-angiogenic effects than CP, in most of assays. J. Cell. Biochem. 117: 2748-2756, 2016. © 2016 Wiley Periodicals, Inc.

• Authors: Marzieh Moeenfard, Alice Cortez, Vera Machado, Raquel Costa, Carla Luís, Pedro Coelho, Raquel Soares, Arminda Alves, Nuno Borges, Alejandro Santos
• Journal: Journal of cellular biochemistry (J Cell Biochem) Vol. 117 Issue 12 Pg. 2748-2756 (12 2016) ISSN: 1097-4644 [Electronic] United States
• PMID: 27129115 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2016 Wiley Periodicals, Inc.

Chemical References

• Angiogenesis Inhibitors
• Diterpenes
• Esters
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• kahweol palmitate
• cafestol palmitate
• KDR protein, human
• Vascular Endothelial Growth Factor Receptor-2

Topics

• Angiogenesis Inhibitors (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Diterpenes (pharmacology)
• Esters (pharmacology)
• Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
• Humans
• Neovascularization, Physiologic (drug effects)
• Phosphorylation (drug effects)
• Tumor Cells, Cultured
• Vascular Endothelial Growth Factor A (metabolism)
• Vascular Endothelial Growth Factor Receptor-2 (metabolism)