p-Coumaric acid, a novel and effective biomarker for quantifying hypoxic stress by HILIC-ESI-MS.

Abstract

In this study, we report p-coumaric acid as novel and effective response marker for indirectly measuring the levels of hypoxia in normal primary bronchial epithelial cells. We developed a simple and rapid technique based on hydrophilic interaction chromatography-electrospray ionization-mass spectrometry (HILIC-ESI-MS). During 168h of hypoxia without induction of reactive oxygen species (ROS), an almost linear increase of p-coumaric acid levels was observed. We interpret the increasing p-coumaric acid concentrations during hypoxia as a result of cell damage, triggered by reduced co-enzyme Q10 levels, because the oxidative cascade was not able to supply sufficient energy. The HILIC-ESI-MS assay within p-coumaric acid exhibited a linear dynamic range from 60 to 610 ng/μL with correlation coefficient of 0.9998. The precision of the assay was ≤15% RSD and method accuracies between 97 and 108%.

• Authors: Yuliya E Silina, Claudia Fink-Straube, Rainer G Hanselmann, Henrike Peuschel, Dietrich A Volmer
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 1020 Pg. 6-13 (May 01 2016) ISSN: 1873-376X [Electronic] Netherlands
• PMID: 27010352 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Actins
• Biomarkers
• Coumaric Acids
• HIF1A protein, human
• Hypoxia-Inducible Factor 1, alpha Subunit
• Propionates
• Ubiquinone
• coenzyme Q10
• p-coumaric acid

Topics

• Actins (analysis, metabolism)
• Biomarkers (analysis, metabolism)
• Cell Hypoxia (physiology)
• Cell Nucleus (chemistry, metabolism)
• Cells, Cultured
• Chromatography, High Pressure Liquid (methods)
• Coumaric Acids (analysis, metabolism)
• Humans
• Hydrophobic and Hydrophilic Interactions
• Hypoxia-Inducible Factor 1, alpha Subunit (analysis, metabolism)
• Oxidative Stress (physiology)
• Propionates
• Reproducibility of Results
• Respiratory Mucosa (cytology)
• Spectrometry, Mass, Electrospray Ionization (methods)
• Ubiquinone (analogs & derivatives)