Abstract | BACKGROUND: METHODS: Bile duct ligation and subsequent daily oral administration of TU-100 was performed in 6-week-old rats. The rats were killed at 3, 7, or 14 days after bile duct ligation to evaluate the liver injury, occurrence of BT, and hepatic fibrosis. As an in vitro experiment, we isolated fresh HSCs from the rats undergoing bile duct ligation. After cell attachment, TU-100 and its 3 component herbs (eg, processed ginger, ginseng radix, and Japanese pepper) were added, and the expressions of Alpha actin2 (acta2), Alpha-1 type I collagen (colIa1), and tissue inhibitor of metalloproteinase 1 (timp1) were analyzed. RESULTS: In vivo experiments demonstrated that oral administration of TU-100 decreased liver injury and atrophy of intestinal mucosa BT, hepatic fibrosis, and hepatic expression of alpha smooth muscle actin (αSMA) and TLR4, compared with rats that underwent bile duct ligation only. In vitro experiments showed that administration of TU-100 or the component herbs inhibited the expressions of acta2, colIa1, and timp1 in the HSCs. CONCLUSION:
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Authors | Keigo Yada, Hiroki Ishibashi, Hiroki Mori, Yuji Morine, Chengzhan Zhu, Rui Feng, Toru Kono, Mitsuo Shimada |
Journal | Surgery
(Surgery)
Vol. 159
Issue 6
Pg. 1600-1611
(06 2016)
ISSN: 1532-7361 [Electronic] United States |
PMID | 26994485
(Publication Type: Journal Article)
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Copyright | Copyright © 2016 Elsevier Inc. All rights reserved. |
Chemical References |
- Actins
- Plant Extracts
- Toll-Like Receptor 4
- dai-kenchu-to
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Topics |
- Actins
(metabolism)
- Animals
- Bacterial Translocation
(drug effects)
- Biliary Atresia
(complications, drug therapy)
- Cell Culture Techniques
- Disease Models, Animal
- Hepatic Stellate Cells
(drug effects)
- Liver Cirrhosis
(etiology, prevention & control)
- Male
- Medicine, Kampo
- Panax
- Phytotherapy
- Plant Extracts
(therapeutic use)
- Rats
- Rats, Wistar
- Toll-Like Receptor 4
(metabolism)
- Zanthoxylum
- Zingiberaceae
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