Cellular cardiomyoplasty into infracted swine's hearts by retrograde infusion through the venous coronary sinus: An experimental study.

Abstract

OBJECTIVES:

The aim was to create a model of myocardial infarction with a borderline myocardial impairment which would enable evaluation of the retrograde cellular cardiomyoplasty through the venous coronary sinus in a large animal model.

MATERIALS AND METHODS:

Fifteen (study group) and 10 juvenile farm pigs (control group) underwent distal left anterior descending artery ligation. One month later the study group animals underwent sternotomy and a murine myoblastic line C2-C12 was injected at a constant pressure of 30mmHg, into the coronary sinus. Thirty days later all animals that survived from both groups underwent transthoracic echocardiography and 99Tc scintigraphy and were later euthanized and specimens were taken for microscopic evaluation.

RESULTS:

Cardiac output decreased significantly after ligation (p<0.001) and increased significantly after cardiomyoplasty (p<0.001). In all animals, the surgical induction of myocardial infarction caused a marked decline in the echocardiographic values of cardiac function; however, the cardiac function and dimensions were significantly improved in the study group after cardiomyoplasty versus the control group. All animals undergoing cardiomyoplasty demonstrated a significant reduction of the perfusion deficit in the left anterior descending artery territory, instead such data remained unchanged in the control group. The histological examination demonstrated the engrafted myoblasts could be distinguished from the activated fibroblasts in the scar tissue because they never showed any signs of collagen secretion and fiber buildup.

CONCLUSIONS:

In conclusion, the venous retrograde delivery route through the coronary sinus is safe and effective, providing a significant improvement in function and viability.

• Authors: Edvin Prifti, Gabriella Di Lascio, Guy Harmelin, Daniele Bani, Vittorio Briganti, Altin Veshti, Massimo Bonacchi
• Journal: Cardiovascular revascularization medicine : including molecular interventions (Cardiovasc Revasc Med) Vol. 17 Issue 4 Pg. 262-71 (Jun 2016) ISSN: 1878-0938 [Electronic] United States
• PMID: 26953214 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Topics

• Animals
• Cardiac Output
• Cardiomyoplasty (methods)
• Cell Line
• Coronary Circulation
• Coronary Sinus
• Disease Models, Animal
• Echocardiography, Doppler
• Female
• Male
• Mice
• Myoblasts (transplantation)
• Myocardial Contraction
• Myocardial Infarction (diagnostic imaging, etiology, physiopathology, surgery)
• Myocardial Perfusion Imaging (methods)
• Myocardium (pathology)
• Recovery of Function
• Swine
• Time Factors
• Tissue Survival
• Tomography, Emission-Computed, Single-Photon
• Ventricular Function, Left