Abstract | PURPOSE: MATERIALS AND METHODS:
Urinary catheters were collected from patients hospitalized for surgical or nonsurgical urological procedures. Catheters were subjected to immunofluorescence analyses by incubation with antifibrinogen antibody and then staining for fluorescence. Fluorescence intensity was compared to that of standard catheters. Catheters were incubated with strains of Enterococcus faecalis, Staphylococcus aureus or Candida to assess binding of those strains to fibrinogen laden catheters. RESULTS: After various surgical and urological procedures, 50 catheters were collected. In vivo dwell time ranged from 1 hour to 59 days. All catheters had fibrinogen deposition. Accumulation depended on dwell time but not on surgical procedure or catheter material. Catheters were probed ex vivo with E. faecalis, S. aureus and Candida albicans, which bound to catheters only in regions where fibrinogen was deposited. CONCLUSIONS:
|
Authors | Ana L Flores-Mireles, Jennifer N Walker, Tyler M Bauman, Aaron M Potretzke, Henry L Schreiber 4th, Alyssa M Park, Jerome S Pinkner, Michael G Caparon, Scott J Hultgren, Alana Desai |
Journal | The Journal of urology
(J Urol)
Vol. 196
Issue 2
Pg. 416-421
(Aug 2016)
ISSN: 1527-3792 [Electronic] United States |
PMID | 26827873
(Publication Type: Journal Article)
|
Copyright | Copyright © 2016 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved. |
Chemical References |
|
Topics |
- Adult
- Bacterial Adhesion
- Biomarkers
(analysis, metabolism)
- Candida albicans
- Catheter-Related Infections
(etiology, microbiology)
- Cross Infection
(etiology, microbiology)
- Enterococcus faecalis
- Female
- Fibrinogen
(analysis, metabolism)
- Humans
- Male
- Staphylococcus aureus
- Urinary Catheterization
(adverse effects)
- Urinary Catheters
(adverse effects, microbiology)
- Urinary Tract Infections
(etiology, microbiology)
- Urologic Surgical Procedures
|