Serum Glycans as Risk Markers for Non-Small Cell Lung Cancer.

Abstract

Previous studies have suggested occurrence of altered serum glycan profiles in patients with lung cancer. Here, we aimed to determine the predictive value of serum glycans to distinguish non-small cell lung cancer (NSCLC) cases from controls in prediagnostic samples using a previously validated predictive protein marker pro-SFTPB, as anchor. Blinded prediagnostic serum samples were obtained from the Carotene and Retinol Efficacy Trial (CARET), and included a discovery set of 100 NSCLC cases and 199 healthy controls. A second test set consisted of 108 cases and 216 controls. Cases and controls were matched for age at baseline (5-year groups), sex, smoking status (current vs. former), study enrollment cohort, and date of blood draw. Serum glycan profiles were determined by mass spectrometry. Twelve glycan variables were identified to have significant discriminatory power between cases and controls in the discovery set (AUC > 0.6). Of these, four were confirmed in the independent validation set. A combination marker yielded AUCs of 0.74 and 0.64 in the discovery and test set, respectively. Four glycan variables exhibited significant incremental value when combined with pro-SFTPB compared with pro-SFTPB alone with AUCs of 0.73, 0.72, 0.72, and 0.72 in the test set, indicating that serum glycan signatures have relevance to risk assessment for NSCLC.

• Authors: L Renee Ruhaak, Carol Stroble, Jianliang Dai, Matt Barnett, Ayumu Taguchi, Gary E Goodman, Suzanne Miyamoto, David Gandara, Ziding Feng, Carlito B Lebrilla, Samir Hanash
• Journal: Cancer prevention research (Philadelphia, Pa.) (Cancer Prev Res (Phila)) Vol. 9 Issue 4 Pg. 317-23 (Apr 2016) ISSN: 1940-6215 [Electronic] United States
• PMID: 26813970 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: ©2016 American Association for Cancer Research.

Chemical References

• Biomarkers, Tumor
• Polysaccharides
• Protein Precursors
• Pulmonary Surfactant-Associated Proteins
• SFTPB protein, human

Topics

• Aged
• Biomarkers, Tumor (blood)
• Carcinoma, Non-Small-Cell Lung (diagnosis)
• Case-Control Studies
• Female
• Humans
• Lung Neoplasms (diagnosis)
• Male
• Mass Spectrometry
• Middle Aged
• Polysaccharides (blood)
• Predictive Value of Tests
• Protein Precursors (blood)
• Pulmonary Surfactant-Associated Proteins (blood)
• Randomized Controlled Trials as Topic
• Risk Assessment
• Risk Factors