Effects of Traumatic Amputation on β-Trace Protein and β2-Microglobulin Concentrations in Male Soldiers.

Abstract

BACKGROUND:

Serum creatinine (SCr) levels are decreased following traumatic amputation, leading to the overestimation of glomerular filtration rate (GFR). β-Trace protein (BTP) and β2-microglobulin (B2M) strongly correlate with measured GFR and have not been studied following amputation. We hypothesized that BTP and B2M would be unaffected by traumatic amputation.

METHODS:

We used the Department of Defense Serum Repository to compare pre- and post-traumatic amputation serum BTP and B2M levels in 33 male soldiers, via the N Latex BTP and B2M nephelometric assays (Siemens Diagnostics, Tarrytown, N.Y., USA). Osterkamp estimation using DEXA scan measurements was used to establish percent estimated body weight loss (%EBWL). Results were analyzed for small (3-5.9% EBWL), medium (6-13.5%), and large (>13.5%) amputation subgroups; and for a control group matched 1:1 to the 12 large amputation subjects. Paired Student's t test was used for comparisons.

RESULTS:

Mean serum BTP levels were unchanged in controls, all amputees, and the small and medium amputation subgroups. BTP appeared to decrease following large %EBWL amputation (p = 0.05). Mean serum B2M levels were unchanged in controls, all amputees, and the small and medium amputation subgroups. B2M appeared to increase following large %EBWL amputation (p = 0.05).

CONCLUSIONS:

BTP and B2M levels are less affected than SCr by amputation, and should be considered for future study of GFR estimation. BTP and B2M changes following large %EBWL amputation require validation and may offer insight into non-GFR BTP and B2M determinants as well as increased cardiovascular disease and mortality following amputation.

• Authors: Dustin J Little, Christina M Yuan, John S Thurlow, Verena Gounden, Sonia Q Doi, Alison Pruziner, Kevin C Abbott, Brett J Theeler, Stephen W Olson
• Journal: American journal of nephrology (Am J Nephrol) Vol. 42 Issue 6 Pg. 436-42 ( 2015) ISSN: 1421-9670 [Electronic] Switzerland
• PMID: 26800100 (Publication Type: Journal Article)
• Copyright: © S. Karger AG, Basel.

Chemical References

• Lipocalins
• beta 2-Microglobulin
• Creatinine
• Intramolecular Oxidoreductases
• prostaglandin R2 D-isomerase

Topics

• Absorptiometry, Photon
• Adolescent
• Adult
• Amputation, Traumatic (blood)
• Body Weight
• Brain Injuries
• Case-Control Studies
• Creatinine (blood)
• Female
• Glomerular Filtration Rate
• Humans
• Intramolecular Oxidoreductases (blood)
• Kidney Function Tests
• Lipocalins (blood)
• Male
• Middle Aged
• Military Personnel
• Models, Statistical
• Nephelometry and Turbidimetry
• Registries
• United States
• Weight Loss
• Young Adult
• beta 2-Microglobulin (blood)