Establishment of a novel method to evaluate peritoneal microdissemination and therapeutic effect using luciferase assay.

Abstract

Peritoneal dissemination is a major cause of recurrence in patients with malignant tumors in the peritoneal cavity. Effective anticancer agents and treatment protocols are necessary to improve outcomes in these patients. However, previous studies using mouse models of peritoneal dissemination have not detected any drug effect against peritoneal micrometastasis. Here we used the luciferase assay to evaluate peritoneal micrometastasis in living animals and established an accurate mouse model of early peritoneal microdissemination to evaluate tumorigenesis and drug efficacy. There was a positive correlation between luminescence intensity in in vivo luciferase assay and the extent of tumor dissemination evaluated by ex vivo luciferase assay and mesenteric weight. This model has advantages over previous models because optimal luciferin concentration without cell damage was validated and peritoneal microdissemination could be quantitatively evaluated. Therefore, it is a useful model to validate peritoneal micrometastasis formation and to evaluate drug efficacy without killing mice.

• Authors: Ryo Takahashi, Takehiko Yokobori, Katsuya Osone, Hironori Tatsuki, Takahiro Takada, Toshinaga Suto, Reina Yajima, Toshihide Kato, Takaaki Fujii, Souichi Tsutsumi, Hiroyuki Kuwano, Takayuki Asao
• Journal: Cancer science (Cancer Sci) Vol. 107 Issue 3 Pg. 341-6 (Mar 2016) ISSN: 1349-7006 [Electronic] England
• PMID: 26716425 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Chemical References

• Antimetabolites, Antineoplastic
• Biomarkers, Tumor
• Deoxycytidine
• Luciferases
• Gemcitabine

Topics

• Animals
• Antimetabolites, Antineoplastic (pharmacology, therapeutic use)
• Biomarkers, Tumor (biosynthesis, genetics)
• Cell Line, Tumor
• Deoxycytidine (analogs & derivatives, pharmacology, therapeutic use)
• Female
• Luciferases (biosynthesis, genetics)
• Mice, Inbred BALB C
• Neoplasm Micrometastasis (diagnosis)
• Neoplasm Transplantation
• Optical Imaging
• Peritoneal Neoplasms (diagnosis, drug therapy, secondary)
• Whole Body Imaging
• Xenograft Model Antitumor Assays
• Gemcitabine