The Safety Evaluation of Salvianolic Acid B and Ginsenoside Rg1 Combination on Mice.

Abstract

Our previous study indicated that the combination of salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1), the main components of Salvia miltiorrhizae and Panax notoginseng, improves myocardium structure and ventricular function in rats with ischemia/reperfusion injury. The present study aimed to determine the safety of the combined SalB and Rg1 (SalB-Rg1) in mice. The safety of SalB-Rg1 was evaluated through acute toxicity and repeated-dose toxicity. In the acute toxicity study, the up and down procedure was carried out firstly, and then, the Bliss method was applied. In the toxicity study for seven-day repeated treatment of SalB-Rg1, forty Kunming mice were randomly divided into four groups. The intravenous median lethal dose (LD50) of the SalB-Rg1 combination was 1747 mg/kg using the Bliss method. For both the acute toxicity study and the seven-day repeated toxicity study, SalB-Rg1 did not induce significant abnormality on brain, heart, kidney, liver and lung structure at any dose based on H&E stain. There were no significant changes related to the SalB-Rg1 toxicity detected on biochemical parameters for two kinds of toxicity studies. The LD50 in mice was 1747 mg/kg, which was more than one hundred times higher than the effective dose. Both studies of acute toxicity and seven-day repeated dose toxicity indicated the safety of the SalB-Rg1 combination.

• Authors: Qun Zhao, Min Yang, Yanping Deng, Haitao Yu, Linlin Wang, Fukang Teng, Kenka Cho, Hongmei Ma, Peng Wu, Xue Li, Wanying Wu, Xuan Liu, Feng Xu, Baohong Jiang, De-An Guo
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 16 Issue 12 Pg. 29345-56 (Dec 09 2015) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 26690140 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzofurans
• Cardiovascular Agents
• Drug Combinations
• Ginsenosides
• salvianolic acid B
• ginsenoside Rg1

Topics

• Animals
• Benzofurans (toxicity)
• Cardiovascular Agents (toxicity)
• Drug Combinations
• Drug Evaluation, Preclinical
• Female
• Ginsenosides (toxicity)
• Lethal Dose 50
• Male
• Mice