Heat-labile enterotoxin of Escherichia coli promotes intestinal colonization of Salmonella enterica.

Abstract

Enterotoxigenic Escherichia coli (ETEC) is an important cause of infantile and travellers' diarrhoea, which poses a serious health burden, especially in developing countries. In addition, ETEC bacteria are a major cause of illness and death in neonatal and recently weaned pigs. The production of a heat-labile enterotoxin (LT) promotes the colonization and pathogenicity of ETEC and may exacerbate co-infections with other enteric pathogens such as Salmonella enterica. We showed that the intraintestinal presence of LT dramatically increased the intestinal Salmonella Typhimurium load in experimentally inoculated pigs. This could not be explained by direct alteration of the invasion or survival capacity of Salmonella in enterocytes, in vitro. However, we demonstrated that LT affects the enteric mucus layer composition in a mucus-secreting goblet cell line by significantly decreasing the expression of mucin 4. The current results show that LT alters the intestinal mucus composition and aggravates a Salmonella Typhimurium infection, which may result in the exacerbation of the diarrhoeal illness.

• Authors: Elin Verbrugghe, Alexander Van Parys, Bregje Leyman, Filip Boyen, Sven Arnouts, Urban Lundberg, Richard Ducatelle, Wim Van den Broeck, Maryam Atef Yekta, Eric Cox, Freddy Haesebrouck, Frank Pasmans
• Journal: Comparative immunology, microbiology and infectious diseases (Comp Immunol Microbiol Infect Dis) Vol. 43 Pg. 1-7 (Dec 2015) ISSN: 1878-1667 [Electronic] England
• PMID: 26616654 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Bacterial Toxins
• Enterotoxins
• Escherichia coli Proteins
• Mucins
• heat-labile enterotoxin, E coli

Topics

• Animals
• Bacterial Load
• Bacterial Toxins (administration & dosage, toxicity)
• Cell Line
• Cell Survival (drug effects)
• Diarrhea (microbiology)
• Disease Models, Animal
• Enterocytes (microbiology)
• Enterotoxigenic Escherichia coli (chemistry)
• Enterotoxins (administration & dosage, toxicity)
• Escherichia coli Proteins (administration & dosage, toxicity)
• Goblet Cells (microbiology)
• Humans
• Intestines (microbiology)
• Jejunum (microbiology)
• Mucins (genetics, metabolism)
• Mucus (chemistry, metabolism)
• Salmonella Infections, Animal (microbiology)
• Salmonella typhimurium (growth & development)
• Swine