Association of polymorphisms in tektin-t gene with idiopathic asthenozoospermia in Sichuan, China.

Abstract

PURPOSE:

The purpose of this research was to study the association between the single nucleotide polymorphisms (SNPs) of the tektin-t gene and idiopathic asthenozoospermia.

METHODS:

We conducted sequence analyses of the tektin-t gene in 104 idiopathic asthenozoospermia and 102 fertile men with normospermic parameters in Sichuan, China.

RESULTS:

In this study, we found that allele 136 T (odds ratio [OR] 1.745, 95 % confidence interval [CI] 1.146-2.655, P = 0.009) was significantly increased in idiopathic asthenozoospermic patients compared with fertile men. This mutation substitutes a highly conserved arginine at position 46 to cysteine. Moreover, PolyPhen-2 analysis predicted that this variant was "probably damaging". In addition, a novel heterozygous mutation, R207H (c.620G >A), was detected in five asthenozoospermic patients, while there was no detection of this genotype among the fertile candidates, indicating that the mutation was located within a conserved domain predicted by PolyPhen-2 analysis as "probably damaging" to the protein.

CONCLUSIONS:

These results suggested that tektin-t variants (Arg/Cys + Cys/Cys) were probably one of the high risk genetic factors for idiopathic asthenozoospermia among males in Sichuan, China, while the R207H polymorphism may be associated with idiopathic asthenozoospermia risk.

• Authors: Shao-hong Zhang, Jian-hui Zhang, Xian-ping Ding, Shun Zhang, Hong-han Chen, Ya-ling Jing
• Journal: Journal of assisted reproduction and genetics (J Assist Reprod Genet) Vol. 33 Issue 2 Pg. 181-7 (Feb 2016) ISSN: 1573-7330 [Electronic] Netherlands
• PMID: 26584823 (Publication Type: Journal Article)

Chemical References

• Microtubule Proteins
• tektins

Topics

• Adult
• Alleles
• Asthenozoospermia (genetics, pathology)
• China
• Genetic Association Studies
• Genotype
• Humans
• Male
• Microtubule Proteins (genetics)
• Mutation
• Polymorphism, Single Nucleotide
• Risk Factors
• Sperm Motility (genetics)
• Spermatozoa (pathology)