Abstract | BACKGROUND: OBJECTIVE: In this study, we aimed to identify and quantify the amount of sulfated glycosaminoglycans (GAG) and hyaluronic acid (HA) within rat femurs following bone drilling and treatment with shock waves. METHODS: To identify and quantify the sulfated glycosaminoglycans (GAG) and hyaluronic acid (HA) within rat femurs following bone drilling and ESWT, 50 male Wistar rats were evaluated. The animals were divided into two groups, both of which were subjected to bone drilling. One of the groups was treated with ESWT. The rats were sacrificed on the 3rd, 7th, 14th, 21st, and 28th day. GAG presence was analyzed by agarose gel electrophoresis with subsequent densitometry and ELISA. RESULTS AND DISCUSSION: The content of sulfated GAGs increased significantly from the 3rd to the 28th day (p = 0.002). Chondroitin sulfate was expressed more highly than the other GAGs. HA content increased significantly at the 3rd day in animals treated with ESWT compared to the control group (p = 0.003). CONCLUSION: ESWT stimulates of sulfated glycosaminoglycans during bone healing and enhanced early expression of HA compared to the control group.
|
Authors | Paulo Roberto Dias dos Santos, Valquiria Pereira De Medeiros, João Paulo Freire Martins de Moura, Carlos Eduardo da Silveira Franciozi, Helena Bonciani Nader, Flavio Faloppa |
Journal | International journal of surgery (London, England)
(Int J Surg)
Vol. 24
Issue Pt B
Pg. 120-3
(Dec 2015)
ISSN: 1743-9159 [Electronic] United States |
PMID | 26432545
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2015 IJS Publishing Group Limited. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
- Glycosaminoglycans
- Hyaluronic Acid
|
Topics |
- Animals
- Glycosaminoglycans
(metabolism)
- High-Energy Shock Waves
- Hyaluronic Acid
(metabolism)
- Male
- Osteogenesis
- Rats, Wistar
|