Complement anaphylatoxin receptors C3aR and C5aR are required in the pathogenesis of experimental autoimmune uveitis.

Abstract

Recent studies have suggested that reagents inhibiting complement activation could be effective in treating T cell mediated autoimmune diseases such as autoimmune uveitis. However, the precise role of the complement anaphylatoxin receptors (C3a and C5a receptors) in the pathogenesis of autoimmune uveitis remains elusive and controversial. We induced experimental autoimmune uveitis in mice deficient or sufficient in both C3a and C5a receptors and rigorously compared their retinal phenotype using various imaging techniques, including indirect ophthalmoscopy, confocal scanning laser ophthalmoscopy, spectral domain optical coherence tomography, topical endoscopic fundus imaging, and histopathological analysis. We also assessed retinal function using electroretinography. Moreover, we performed Ag-specific T cell recall assays and T cell adoptive transfer experiments to compare pathogenic T cell activity between wild-type and knockout mice with experimental autoimmune uveitis. These experiments showed that C3a receptor/C5a receptor-deficient mice developed much less severe uveitis than did control mice using all retinal examination methods and that these mice had reduced pathogenic T cell responses. Our data demonstrate that both complement anaphylatoxin receptors are important for the development of experimental autoimmune uveitis, suggesting that targeting these receptors could be a valid approach for treating patients with autoimmune uveitis.

• Authors: Lingjun Zhang, Brent A Bell, Minzhong Yu, Chi-Chao Chan, Neal S Peachey, John Fung, Xiaoming Zhang, Rachel R Caspi, Feng Lin
• Journal: Journal of leukocyte biology (J Leukoc Biol) Vol. 99 Issue 3 Pg. 447-54 (Mar 2016) ISSN: 1938-3673 [Electronic] England
• PMID: 26394814 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © Society for Leukocyte Biology.

Chemical References

• C5ar1 protein, mouse
• Receptor, Anaphylatoxin C5a
• Receptors, Complement
• complement C3a receptor

Topics

• Adoptive Transfer
• Animals
• Autoimmune Diseases (etiology)
• Electroretinography
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Receptor, Anaphylatoxin C5a (physiology)
• Receptors, Complement (physiology)
• Th1 Cells (immunology)
• Th17 Cells (immunology)
• Uveitis (etiology)