Abstract |
Intrauterine adhesions (IUAs), which are characterized by endometrial fibrosis, increase the risk of secondary infertility and recurrent miscarriage. MicroRNA-29 (miR-29) is a potent inhibitor of TGF-β1/Smad signaling. In this study, we investigated the therapeutic potential of agomir-29b, an miR-29b mimic, in endometrial fibrosis induced by dual injury (uterine curettage and lipopolysaccharide treatment) in a rat model of IUA and explored the underlying mechanism. We found that injured rats developed endometrial fibrosis characterized by increased COL1A1 and α-smooth muscle actin expression and decreased E-cadherin expression, associated with a loss of miR-29b. Overexpression of miR-29b before injury prevented endometrial fibrosis including collagen accumulation and epithelial-mesenchymal transition. Delay of agomir-29b treatment until endometrial fibrosis was established on day 4 also halted the progression of disease. Further experiments indicated that miR-29b inhibited endometrial fibrosis via blockade of the Sp1-TGF-β1/Smad-CTGF pathway. In conclusion, agomir-29b may act as a novel and effective therapeutic agent against IUAs.
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Authors | Jingxiong Li, Shaohua Du, Xiujie Sheng, Juan Liu, Bohong Cen, Feng Huang, Yuanli He |
Journal | Reproductive sciences (Thousand Oaks, Calif.)
(Reprod Sci)
Vol. 23
Issue 3
Pg. 386-94
(Mar 2016)
ISSN: 1933-7205 [Electronic] United States |
PMID | 26392347
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Author(s) 2015. |
Chemical References |
- CCN2 protein, rat
- MIRN29 microRNA, mouse
- MicroRNAs
- Smad Proteins
- Sp1 Transcription Factor
- SP1 protein, human
- Transforming Growth Factor beta1
- Connective Tissue Growth Factor
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Topics |
- Animals
- Connective Tissue Growth Factor
(physiology)
- Disease Models, Animal
- Endometrium
(metabolism, pathology)
- Female
- Fibrosis
(metabolism, pathology)
- MicroRNAs
(biosynthesis)
- Rats
- Rats, Sprague-Dawley
- Smad Proteins
(physiology)
- Sp1 Transcription Factor
(physiology)
- Transforming Growth Factor beta1
(physiology)
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