A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.

Abstract

Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.

• Authors: Edoardo Petrini, Victoire Baillet, Jake Cridge, Cassandra J Hogan, Cindy Guillaume, Huiling Ke, Elisa Brandetti, Simon Walker, Hashem Koohy, Mikhail Spivakov, Patrick Varga-Weisz
• Journal: Journal of cell science (J Cell Sci) Vol. 128 Issue 20 Pg. 3707-13 (Oct 15 2015) ISSN: 1477-9137 [Electronic] England
• PMID: 26345368 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015. Published by The Company of Biologists Ltd.

Chemical References

• Actin-Related Protein 2
• Phosphates
• Schizosaccharomyces pombe Proteins
• Sla2 protein, S pombe
• myo1 protein, S pombe
• Myosin Heavy Chains

Topics

• Actin-Related Protein 2 (genetics, metabolism)
• Endocytosis (physiology)
• Gene Deletion
• Gene Expression Regulation, Fungal (physiology)
• Myosin Heavy Chains (genetics, metabolism)
• Phosphates (metabolism)
• Schizosaccharomyces (genetics, metabolism)
• Schizosaccharomyces pombe Proteins (genetics, metabolism)
• Signal Transduction (physiology)
• Transcriptome