Abstract | BACKGROUND: Human Rhinovirus (HRV) is responsible for the majority of common colds and is frequently accompanied by secondary bacterial infections through poorly understood mechanisms. We investigated the effects of experimental human HRV serotype 16 infection on the upper respiratory tract microbiota. METHODS: Six healthy volunteers were infected with HRV16. We performed 16S ribosomal RNA-targeted pyrosequencing on throat swabs taken prior, during and after infection. We compared overall community diversity, phylogenetic structure of the ecosystem and relative abundances of the different bacteria between time points. RESULTS: During acute infection strong trends towards increases in the relative abundances of Haemophilus parainfluenzae and Neisseria subflava were observed, as well as a weaker trend towards increases of Staphylococcus aureus. No major differences were observed between day-1 and day 60, whereas differences between subjects were very high. CONCLUSIONS:
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Authors | J J Hofstra, S Matamoros, M A van de Pol, B de Wever, M W Tanck, H Wendt-Knol, M Deijs, L van der Hoek, K C Wolthers, R Molenkamp, C E Visser, P J Sterk, R Lutter, M D de Jong |
Journal | BMC infectious diseases
(BMC Infect Dis)
Vol. 15
Pg. 336
(Aug 14 2015)
ISSN: 1471-2334 [Electronic] England |
PMID | 26271750
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Adult
- Female
- Haemophilus parainfluenzae
(isolation & purification)
- Humans
- Male
- Microbiota
- Middle Aged
- Neisseria
(isolation & purification)
- Pharynx
(microbiology)
- Picornaviridae Infections
(microbiology)
- RNA, Ribosomal, 16S
(analysis)
- Respiratory Tract Infections
(microbiology)
- Rhinovirus
- Staphylococcus aureus
(isolation & purification)
- Young Adult
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