HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Therapeutic effect of the YH6 phage in a murine hemorrhagic pneumonia model.

Abstract
The treatment, in farmed mink, of hemorrhagic pneumonia caused by multidrug-resistant Pseudomonas aeruginosa strains has become increasingly difficult. This study investigated the potential use of phages as a therapy against hemorrhagic pneumonia caused by P. aeruginosa in a murine hemorrhagic pneumonia model. An N4-like phage designated YH6 was isolated using P. aeruginosa strain D9. YH6 is a virulent phage with efficient and broad host lytic activity against P. aeruginosa. No bacterial virulence- or lysogenesis-related ORF is present in the YH6 genome, making it eligible for use in phage therapy. In our murine experiments, a single intranasal administration of YH6 (2 × 10(7) PFU) 2 h after D9 intranasal injections at double minimum lethal dose was sufficient to protect mice against hemorrhagic pneumonia. The bacterial load in the lungs of YH6-protected mice was less than 10(3) CFU/g within 24 h after challenge and ultimately became undetectable, whereas the amount of bacteria in the lung tissue derived from unprotected mice was more than 10(8) CFU/g within 24 h after challenge. In view of its protective efficacy in this murine hemorrhagic pneumonia model, YH6 may serve as an alternative treatment strategy for infections caused by multidrug-resistant P. aeruginosa.
AuthorsMei Yang, Chongtao Du, Pengjuan Gong, Feifei Xia, Changjiang Sun, Xin Feng, Liancheng Lei, Jun Song, Lei Zhang, Bin Wang, Feng Xiao, Xinwu Yan, Ziyin Cui, Xinwei Li, Jingmin Gu, Wenyu Han
JournalResearch in microbiology (Res Microbiol) Vol. 166 Issue 8 Pg. 633-43 (Oct 2015) ISSN: 1769-7123 [Electronic] France
PMID26254772 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Topics
  • Administration, Intranasal
  • Animals
  • Bacterial Load
  • Biological Therapy
  • Disease Models, Animal
  • Drug Resistance, Multiple, Bacterial
  • Female
  • Lung (microbiology, pathology)
  • Mice
  • Pneumonia, Bacterial (microbiology, therapy)
  • Pseudomonas Infections (immunology, microbiology, therapy)
  • Pseudomonas Phages (isolation & purification, physiology)
  • Pseudomonas aeruginosa (pathogenicity, virology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: