Abstract |
The treatment, in farmed mink, of hemorrhagic pneumonia caused by multidrug-resistant Pseudomonas aeruginosa strains has become increasingly difficult. This study investigated the potential use of phages as a therapy against hemorrhagic pneumonia caused by P. aeruginosa in a murine hemorrhagic pneumonia model. An N4-like phage designated YH6 was isolated using P. aeruginosa strain D9. YH6 is a virulent phage with efficient and broad host lytic activity against P. aeruginosa. No bacterial virulence- or lysogenesis-related ORF is present in the YH6 genome, making it eligible for use in phage therapy. In our murine experiments, a single intranasal administration of YH6 (2 × 10(7) PFU) 2 h after D9 intranasal injections at double minimum lethal dose was sufficient to protect mice against hemorrhagic pneumonia. The bacterial load in the lungs of YH6-protected mice was less than 10(3) CFU/g within 24 h after challenge and ultimately became undetectable, whereas the amount of bacteria in the lung tissue derived from unprotected mice was more than 10(8) CFU/g within 24 h after challenge. In view of its protective efficacy in this murine hemorrhagic pneumonia model, YH6 may serve as an alternative treatment strategy for infections caused by multidrug-resistant P. aeruginosa.
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Authors | Mei Yang, Chongtao Du, Pengjuan Gong, Feifei Xia, Changjiang Sun, Xin Feng, Liancheng Lei, Jun Song, Lei Zhang, Bin Wang, Feng Xiao, Xinwu Yan, Ziyin Cui, Xinwei Li, Jingmin Gu, Wenyu Han |
Journal | Research in microbiology
(Res Microbiol)
Vol. 166
Issue 8
Pg. 633-43
(Oct 2015)
ISSN: 1769-7123 [Electronic] France |
PMID | 26254772
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. |
Topics |
- Administration, Intranasal
- Animals
- Bacterial Load
- Biological Therapy
- Disease Models, Animal
- Drug Resistance, Multiple, Bacterial
- Female
- Lung
(microbiology, pathology)
- Mice
- Pneumonia, Bacterial
(microbiology, therapy)
- Pseudomonas Infections
(immunology, microbiology, therapy)
- Pseudomonas Phages
(isolation & purification, physiology)
- Pseudomonas aeruginosa
(pathogenicity, virology)
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