Abstract | AIMS: This study aims to evaluate the anti- cancer effect of daucosterol and explore its possible mechanism. MAIN METHODS: MTT and colony formation assay were performed to determine the effect of daucosterol on cancer cell proliferation in vitro. H22 allograft model was used for the assessment of its anti- cancer activity in vivo. Intracellular generation of reactive oxygen species (ROS) was measured using DCFH-DA probe with flow cytometry system and a laser scanning confocal microscope. LC3 ( microtubule-associated protein 1 light chain 3)-II conversion was monitored with immunofluorescence and immunoblotting to demonstrate daucosterol-induced autophagy. KEY FINDINGS: SIGNIFICANCE:
Daucosterol inhibits cancer cell proliferation by inducing autophagy through ROS-dependent manner and could be potentially developed as an anti- cancer agent.
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Authors | Chuanke Zhao, Tiantian She, Lixin Wang, Yahui Su, Like Qu, Yujing Gao, Shuo Xu, Shaoqing Cai, Chengchao Shou |
Journal | Life sciences
(Life Sci)
Vol. 137
Pg. 37-43
(Sep 15 2015)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 26209138
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Reactive Oxygen Species
- Sitosterols
- 3-methyladenine
- Glutathione
- Adenine
- lyoniside
- Acetylcysteine
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Topics |
- Acetylcysteine
(pharmacology)
- Adenine
(analogs & derivatives, pharmacology)
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Autophagy
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Dose-Response Relationship, Drug
- Drug Screening Assays, Antitumor
- Glutathione
(pharmacology)
- Humans
- Mice
- Neoplasms
(drug therapy, metabolism, pathology)
- Reactive Oxygen Species
(metabolism)
- Sitosterols
(antagonists & inhibitors, pharmacology)
- Tumor Stem Cell Assay
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