Effects of daidzein in regards to cytotoxicity in vitro, apoptosis, reactive oxygen species level, cell cycle arrest and the expression of caspase and Bcl-2 family proteins.

Abstract

In the present study, the in vitro cytotoxicity of daidzein was evaluated in human BEL-7402, A549, HeLa, HepG-2 and MG-63 cancer cell lines. BEL-7402 cells were sensitive to daidzein treatment, with an IC50 value of 59.7±8.1 µM. Daidzein showed no cytotoxic activity toward A549, HeLa, HepG-2 and MG-63 cells. Daidzein increased the levels of reactive oxygen species (ROS) and induced a decrease in mitochondrial membrane potential. Morphological and comet assays showed that daidzein effectively induced apoptosis in BEL-7402 cells. Additionally, daidzein caused cell cycle arrest at the G2/M phase in the BEL-7402 cell line. Daidzein downregulated the expression of Bcl-2, Bcl-x and Baid proteins and upregulated the levels of Bim protein in the BEL-7402 cells. The results demonstrated that daidzein induced BEL-7402 cell apoptosis through an ROS-mediated mitochondrial dysfunction pathway.

• Authors: Bing-Jie Han, Wei Li, Guang-Bin Jiang, Shang-Hai Lai, Cheng Zhang, Chuan-Chuan Zeng, Yun-Jun Liu
• Journal: Oncology reports (Oncol Rep) Vol. 34 Issue 3 Pg. 1115-20 (Sep 2015) ISSN: 1791-2431 [Electronic] Greece
• PMID: 26178389 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Isoflavones
• Phytoestrogens
• Proto-Oncogene Proteins c-bcl-2
• Reactive Oxygen Species
• daidzein
• Caspases

Topics

• Apoptosis (drug effects)
• Blotting, Western
• Caspases (biosynthesis)
• Cell Cycle Checkpoints (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Comet Assay
• Flow Cytometry
• Humans
• In Vitro Techniques
• Isoflavones (pharmacology)
• Membrane Potential, Mitochondrial (drug effects)
• Phytoestrogens (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (biosynthesis)
• Reactive Oxygen Species (metabolism)