Regulation of BAX/BCL2 gene expression in breast cancer cells by docetaxel-loaded human serum albumin nanoparticles.

Abstract

Today, using nanoparticle-based drug delivery systems has expanded to avoid anticancer side effects. Taxanes are important chemotherapeutic agents in the treatment of metastatic breast cancer. In this study, docetaxel (DTX)-loaded human serum albumin (HSA) nanoparticles (NPs) were prepared and characterized. Drug toxicity of the nanoparticles was measured by MTT assay with different drug concentrations (0.01, 0.1, 0.5, 1 and 5 μM) at different incubation times (24, 48 and 72 h). Expression of BAX/BCL2 mRNA levels was determined by real-time PCR. The size of NPs prepared and used in our study was about 147 nm with surface charge of -29.6 mV. Results obtained from MTT assay showed that 0.5 μM of free drug had 50 % toxicity on MCF-7 cells after 48-h incubation. Real-time PCR results showed an increase in expression of BAX and no change for BCL2. In conclusion, a significant overexpression of BAX gene and changes in BAX/BCL2 ratio were observed for DTX-loaded HSA nanoparticles compared with free DTX and may provide a potential therapy to inhibit anticancer drug resistance.

• Authors: Marzieh Kordezangeneh, Shiva Irani, Reza Mirfakhraie, Mehdi Esfandyari-Manesh, Fatemeh Atyabi, Rassoul Dinarvand
• Journal: Medical oncology (Northwood, London, England) (Med Oncol) Vol. 32 Issue 7 Pg. 208 (Jul 2015) ISSN: 1559-131X [Electronic] United States
• PMID: 26099171 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• BAX protein, human
• BCL2 protein, human
• Proto-Oncogene Proteins c-bcl-2
• RNA, Messenger
• Serum Albumin
• Taxoids
• bcl-2-Associated X Protein
• Docetaxel

Topics

• Antineoplastic Agents (administration & dosage)
• Breast Neoplasms (drug therapy, genetics)
• Cell Line, Tumor
• Docetaxel
• Female
• Gene Expression (drug effects)
• Humans
• MCF-7 Cells
• Nanoparticles (administration & dosage)
• Proto-Oncogene Proteins c-bcl-2 (genetics)
• RNA, Messenger (genetics)
• Serum Albumin (administration & dosage)
• Taxoids (administration & dosage)
• bcl-2-Associated X Protein (genetics)