GLU298ASP and 4G/5G Polymorphisms and the Risk of Ischemic Stroke in Young Individuals.

Abstract

BACKGROUND:

Polymorphisms in the endothelial nitric oxide synthase (eNOS) and in the plasminogen activator inhibitor -1 (PAI-1) genes have been implicated in stroke pathogenesis but results are still controversial. The aim of this study was to examine the possible contribution of Glu298Asp in the eNOS and 4G/5G in the PAI-1polymorphisms with ischemic stroke in a young Mexican population.

MATERIALS AND METHODS:

In a case-control study, conducted between January 2006 and June 2010, 204 patients ≤45 years of age with ischemic stroke and 204 controls matched by age and gender, were recruited. The Glu298Asp and 4G/5G polymorphisms were determined in all participants by polymerase chain reaction-restriction fragment length polymorphism.

RESULTS:

There was a significant difference in the Glu298Asp genotype distribution (P=0.001) and allele frequency between the two groups (P=0.001). The 4G/5G genotype distribution (P=0.40) and the allele frequency was similar between groups; (P=0.13). There were independent factors for ischemic stroke: Asp carriage (GluAsp+AspAsp) (P=0.02); smoking (P=0.01); hypertension (P=0.03), and familial history of atherothrombotic disease (P=0.04).

CONCLUSIONS:

The Asp allele from the Gu298Asp gene represents an independent risk factor for ischemic stroke in a young Mexican population. In contrast, the 4G/5G was not associated with an increased risk for this disease in the same group of patients, as previously has been demonstrated in other populations.

• Authors: Juan Carlos Esparza-García, David Santiago-Germán, María Guadalupe Valades-Mejía, Jesus Hernández-Juárez, Eberth Aguilar-Sosa, Alfredo Leaños-Miranda, Antonio Alvarado-Moreno, Abraham Majluf-Cruz, Irma Isordia-Salas
• Journal: The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques (Can J Neurol Sci) Vol. 42 Issue 5 Pg. 310-6 (Sep 2015) ISSN: 0317-1671 [Print] England
• PMID: 26036781 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plasminogen Activator Inhibitor 1
• Aspartic Acid
• Glutamic Acid
• Nitric Oxide Synthase Type III

Topics

• Adult
• Aspartic Acid (genetics)
• Brain Ischemia (complications)
• Case-Control Studies
• DNA Mutational Analysis
• Female
• Gene Frequency
• Genetic Predisposition to Disease (genetics)
• Genotype
• Glutamic Acid (genetics)
• Humans
• Male
• Mexico
• Nitric Oxide Synthase Type III (genetics)
• Plasminogen Activator Inhibitor 1 (genetics)
• Polymorphism, Genetic (genetics)
• Stroke (etiology, genetics)