miR-25 targets the modulator of apoptosis 1 gene in lung cancer.

Abstract

To determine the role of miR-25 in non-small cell lung cancer (NSCLC), we first detected miR-25 expression in clinical specimens and lung cancer cell lines by quantitative real-time polymerase chain reaction. The levels of miR-25 were elevated in the plasma of NSCLC patients and NSCLC cell lines. Transfection of A549 and 95-D cells with a miR-25 inhibitor resulted in reduced cell proliferation and enhanced apoptosis. Moreover, the modulator of apoptosis 1 (MOAP1) gene was identified as a novel target of miR-25. The ability of miR-25 to promote cell proliferation and block apoptosis is attributable to its effect on MOAP1 suppression. In addition, miR-25 antagomir significantly inhibited lung cancer growth via upregulation of MOAP1 in a mouse xenograft model. Collectively, these data demonstrate that miR-25 is an important biomarker for lung cancer, and miR-25 promotes cell proliferation and inhibits apoptosis in NSCLC cells by negatively regulating MOAP1 expression.

• Authors: Tangwei Wu, Weiqun Chen, Deyong Kong, Xiaoyi Li, Hongda Lu, Shuiyi Liu, Jing Wang, Lili Du, Qingzhi Kong, Xiaodong Huang, Zhongxin Lu
• Journal: Carcinogenesis (Carcinogenesis) Vol. 36 Issue 8 Pg. 925-35 (Aug 2015) ISSN: 1460-2180 [Electronic] England
• PMID: 25998847 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected].

Chemical References

• Adaptor Proteins, Signal Transducing
• Apoptosis Regulatory Proteins
• MIRN25 microRNA, human
• MOAP1 protein, human
• MicroRNAs

Topics

• Adaptor Proteins, Signal Transducing (genetics)
• Aged
• Animals
• Apoptosis (genetics)
• Apoptosis Regulatory Proteins (genetics)
• Carcinoma, Non-Small-Cell Lung (genetics, pathology)
• Case-Control Studies
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, pathology)
• Male
• Mice, Inbred BALB C
• MicroRNAs (blood, genetics)
• Middle Aged
• Xenograft Model Antitumor Assays