Inactivation of Rab23 inhibits the invasion and motility of pancreatic duct adenocarcinoma.

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is charac-terized by a poor prognosis and high mortality rate. In this study, we investigated the expression of Rab23 in non-tumor pancreatic tissues and PDACs via immunohistochemistry. Rab23 was found in 39 of 58 (67.2%) and in 11 of 30 (36.7%) of the PDAC and non-tumor pan-creatic tissue samples (P = 0.0073), respectively. There were signifi-cant correlations between Rab23 expression and unfavorable variables, including cancer differentiation level (P = 0.0089), lymph nodal (P = 0.0099), and distant metastases (P = 0.0173). Inactivation with small interfering RNA against Rab23 in the human pancreatic cancer cell line Panc-1 inhibited the migration and invasive potential of the cells. Our data provide new insight into the essential role of Rab23 in PDAC inva-sion and metastasis and suggest that Rab23 expression is a useful indi-cator of metastatic potential; hence, it may be a new therapeutic target for this common malignancy.

• Authors: Z Z Cai, L B Xu, J L Cai, J S Wang, B Zhou, H Hu
• Journal: Genetics and molecular research : GMR (Genet Mol Res) Vol. 14 Issue 1 Pg. 2707-15 (Mar 30 2015) ISSN: 1676-5680 [Electronic] Brazil
• PMID: 25867419 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RAB23 protein, human
• rab GTP-Binding Proteins

Topics

• Blotting, Western
• Carcinoma, Pancreatic Ductal (genetics, metabolism, pathology)
• Cell Line
• Cell Movement (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Neoplasm Invasiveness
• Pancreatic Neoplasms (genetics, metabolism, pathology)
• RNA Interference
• Reverse Transcriptase Polymerase Chain Reaction
• rab GTP-Binding Proteins (genetics, metabolism)