Hypoacetylation in association with histone 3 modulation in human hepatocellular carcinoma.

Abstract

BACKGROUND:

Aberrant histone deacetylase expression may cause imbalance between acetylation and deacetylation of histone and play roles in tumor transformation. We found that histone 3 was modulated in human hepatocellular carcinoma. We determined if histone 3 modulation is related to the aberrant expression of histone deacetylase.

MATERIALS AND METHODS:

We analyzed human liver and hepatocellular carcinoma tissues and fibroblast and fibrosarcoma cell lines for the expression of histone 3, histone deacetylase 1 and acetylated histone 3 using immunohistochemistry, western blot and immunofluorescence.

RESULTS:

Histone deacetylase 1 and histone 3 were more strongly detected in hepatocellular carcinoma tissue and fibrosarcoma cells than in liver tissues and fibroblast cells, respectively. However, acetylated histone 3 was more strongly expressed in normal liver and fibroblast cells and less expressed in hepatocellular carcinoma and fibrosarcoma cells.

CONCLUSION:

Histone deacetylase 1 overexpression and hypoacetylation of histone 3 might play critical roles in the modulation of histone 3 in human hepatocellular carcinoma.

• Authors: Chiung-Chi Cheng, Yi-Hsiang Liu, Yen-Chang Clark Lai, Yung-Hsiang Hsu, Wei-Ting Chao, Yih-Shyong Lai
• Journal: In vivo (Athens, Greece) (In Vivo) 2015 Mar-Apr Vol. 29 Issue 2 Pg. 237-42 ISSN: 1791-7549 [Electronic] Greece
• PMID: 25792651 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• Histones
• Histone Deacetylase 1

Topics

• Acetylation
• Carcinoma, Hepatocellular (genetics, metabolism, pathology)
• Cell Line, Tumor
• Histone Deacetylase 1 (genetics, metabolism)
• Histones (metabolism)
• Humans
• Immunohistochemistry
• Liver Neoplasms (genetics, metabolism, pathology)